山东科学2017，Vol.30Issue(4)：106-111,123,7.DOI:10.3976/j.issn.1002-4026.2017.04.017
基于原子力显微镜研究p53蛋白与PBR322 DNA的相互作用
Study on interaction between p53 protein and PBR322 DNA based on atomic force microscopy
摘要
Abstract
Tumor (cancer) is caused by the apoptosis or proliferation of cells when DNA damage cannot be repaired in time in the process of replication.The processes of DNA transcription and expression are all involved in the interaction of protein and DNA in living cells.And, tumor suppressor protein is also one of the key proteins involved in this process.However, many studies have found that the tumor suppressor protein p53 can recognize and repair damaging-DNA, which plays a significant role in cell apoptosis, gene protection and avoidance of cancer.Studies have shown that the structural stability of p53 protein and the affinity of p53-DNA can be enhanced by metal ions, such as magnesium and zinc ions.Therefore, based on atomic force microscopy (AFM), the interaction image of p53 protein and PBR322 ring DNA is visually presented.And, it is found that p53 protein can promote a ring DNA aggregation or cross in itself.However, this phenomenon occurrence is rare for linear DNA with the length of 5 000bp, and it is not occurrence in 20 000bp DNA either.However, protein p53 can make ring DNA to be a twist shape in high magnesium concentration solution, that is, the supercoil shape is formed.This phenomenon may provide guidance for the study on the function and mechanism of protein p53, as well as provide insights into cancer therapy, cancer drug development and cancer detection methods.关键词
p53蛋白/金属镁离子/PBR322环状DNA/相互作用/DNA超螺旋Key words
protein p53/metal magnesium ions/PBR322 ring DNA/interaction/DNA supercoil分类
生物科学引用本文复制引用
陈洋,王艳伟,杨光参..基于原子力显微镜研究p53蛋白与PBR322 DNA的相互作用[J].山东科学,2017,30(4):106-111,123,7.基金项目
国家自然科学基金(11274245, 10974146, 11304232) （11274245, 10974146, 11304232）
温州大学创新基金(3162015033) （3162015033）
