山东农业大学学报（自然科学版）2017，Vol.48Issue(4)：537-539,548,4.DOI:10.3969/j.issn.1000-2324.2017.04.012
铁皮石斛组培体系的建立
Tissue Culture System Establishment of Dendrobium officinale Kimura et Migo
郑子首 1孙晨瑜 1吕晓倩 1侯文秋 1李滨1
作者信息
- 1. 山东农业大学 生命科学学院, 山东 泰安 271018
- 折叠
摘要
Abstract
As an important medicine for anticancer, Dendrobium officinale Kimura et Migo becomes a hot topic during thehealth-preserving society. Wild Dendrobium officinale is very rare and very expensive and not suitable for publicconsumption. So the establishment of the tissue culture system suitable for industrial production has become the tendency.We used its stalk as material and 1/2 MS as basal culture medium, investigated the effects of different hormone concentrationand other factors on the tissue culture of D. Officinale, and then used these plants cultivated as material to explore the effectsof temperature, water quality, culture medium and watering method on the seedling of Dendrobium officinale. The resultsshowed that the best medium of rapid multiplication was basal culture medium+1.0 mg/L 6-BA + 1.0 mg/LNAA+1% PAC,and it's inductivity was 12.5%. The best medium of tissue culture was basal culture medium+1.5 mg/L 6-BA + 2.0 mg/LNAA or 0.5 mg/L 6-BA + 0.5 mg/L NAA or 0.5 mg/L NAA + 1.5 mg/L 2,4-D, and their inductivities were all over 80%. Thebest conditions to hardening-seedlings was 25℃+lighted tap wate, the most suitable culture medium was sphagna+ 0.5~1.0cm barks in diameter.关键词
铁皮石斛/组培Key words
Dendrobium officinale Kimura et Migo/tissue culture分类
农业科技引用本文复制引用
郑子首,孙晨瑜,吕晓倩,侯文秋,李滨..铁皮石斛组培体系的建立[J].山东农业大学学报（自然科学版）,2017,48(4):537-539,548,4.
