山东农业大学学报（自然科学版）2017，Vol.48Issue(4)：545-548,4.DOI:10.3969/j.issn.1000-2324.2017.04.014
不同调控因子下GUS基因在马铃薯块茎中瞬时表达的影响
The Effects of Different Regulatory Factors on Transient Expression of GUS Gene in Potato Tubers
舒锐 1姚甜甜 2李燕 1臧传江 1焦健 1兰成云 1刘少军1
作者信息
- 1. 山东省轻工农副原料研究所, 山东 高密 261500
- 2. 山东农业大学 园艺科学与工程学院, 山东 泰安 271018
- 折叠
摘要
Abstract
The GUS gene was transiently expressed in potato tubers by agrobacterium-mediated transformation and the expression the GUS was detected by histochemical staining. The effects of acetyligenone concentration, vacuum intensity, immersion time, and total culture time on the expression of GUS gene was investigated. The results show that the different vacuum strength and co-culture time exhibit the notable influence on GUS expression and the expression levels are higher when the vacuum intensity is 1kPa and the co-culture time is 2 days.关键词
马铃薯/GUS/调控因子/瞬时表达Key words
Potato/GUS/regulatory factors/transient expression分类
农业科技引用本文复制引用
舒锐,姚甜甜,李燕,臧传江,焦健,兰成云,刘少军..不同调控因子下GUS基因在马铃薯块茎中瞬时表达的影响[J].山东农业大学学报（自然科学版）,2017,48(4):545-548,4.
