山东农业大学学报（自然科学版）2017，Vol.48Issue(4)：556-561,6.DOI:10.3969/j.issn.1000-2324.2017.04.017
一株产纤维素酶菌株的鉴定及其产酶条件优化
Identification for a Strain of Produce Cellulase and Enzyme Production Conditions Optimization
摘要
Abstract
This paper screened a strain of cellulose-producing bacteria No. X-2 from the soil. It was an aerobic Gram-positive bacteria and able to generate spore. It had been identified as Bacillus thuringiensis in laboratory according to thallus morphology, Colony morphology, physiological and biochemical characteristics and 16S rDNA molecular biology identification. Liquid fermentation test showed that Optimum fermentation conditions of X-2 were 72 h fermentation time, incubation temperature at 35 ℃, 130 rpm of revolving speed, 6%of inoculum size and pH7.2 of the initial value. The activity of hydroxymethyl cellulase (CMCase) under this condition reached 10.89 U/mL.关键词
纤维素酶/鉴定/苏云金芽孢杆菌/产酶条件优化Key words
Cellulase/identification/Bacillus thuringiensis/optimization of fermentation conditions分类
生物科学引用本文复制引用
李杨,桓明辉,高晓梅,刘晓辉,敖静,朱巍巍,池景良..一株产纤维素酶菌株的鉴定及其产酶条件优化[J].山东农业大学学报（自然科学版）,2017,48(4):556-561,6.基金项目
国家农业科技成果转化资金项目(2013GB2B000093) （2013GB2B000093）
