生物骨科材料与临床研究2017，Vol.14Issue(4)：15-19,23,后插2,7.DOI:10.3969/j.issn.1672-5972.2017.04.004
玻璃酸钠对兔骨关节炎早期iNOS炎症途径及炎性细胞因子表达影响的实验研究
Experimental study of sodium hyaluronate on the expression of iNOS and inflammatory cytokines in rabbit osteoarhitis
摘要
Abstract
Objective To investigate the correlation between the production of NO,iNOS and the expressions of TNF-α、IL-1β、IL-6 and IL-17 in the articular cartilage of rabbits with traumatic osteoarthritis (OA),and to explore the influence of Sodium hyaluronate (SH) on cartilage degeneration in traumatic OA.Methods 48 New Zealand rabbits underwent anterior cruciateligament transaction (ACLT) of the right knee and then were divided into three groups.The animals in the SH-treatment group were injected with SH (0.3 mg) in the knees 4 week after operation,once a week for 4 weeks.Rabbits in the model group were treated with normal saline under the same condition and the control group does nothing.All rabbits were sacrificed after the last treatment.Cartilagineus changes ofthe medial femoral condyles were grossly checked and OA severity was evaluated under light-microscope-The concentration of NO and iNOS in joint fluid,serum and the expressions of TNF-α、IL-1 β、IL-6 and IL-17 in joint fluid were assessed ELISA kit.Results The results of HE staining and pathological score show that cartilage degeneration in the SH-treatment group was significantly slighter than that in the model group.The concentration of NO and iNOS in joint fluid,serum and the expressions of TNF-α、IL-1β 、IL-6 and IL-17 in joint fluid were significantly lower than those in the model group (P＜0.01) after SH treatment.Conclusion The expression of NO was positively correlated with inflammatory Cytokines in the early stages of OA.And SH may protect articular cartilage by inhibiting synthesis of NO.关键词
骨关节炎/玻璃酸钠/一氧化氮/细胞因子Key words
Osteoarthritis/Sodium hyaluronate/NO/Cytokines分类
医药卫生引用本文复制引用
韦卓,袁冰,马琴,刘平,姚弘毅,阮锋..玻璃酸钠对兔骨关节炎早期iNOS炎症途径及炎性细胞因子表达影响的实验研究[J].生物骨科材料与临床研究,2017,14(4):15-19,23,后插2,7.基金项目
湖北省卫生和计划生育委员会科研项目立项(WJ2015MB018) （WJ2015MB018）
