天津中医药2017，Vol.34Issue(8)：554-558,5.DOI:10.11656/j.issn.1672-1519.2017.08.16
复方红景天对大鼠缺血性脑损伤后海马组织细胞凋亡的影响
Study on effect of Compound Rhodiola on apoptosis of hippocampus in rats with cerebral ischemia
摘要
Abstract
[Objective] To study the effect of Compound Rhodiola on apoptosis of hippocampus cells after ischemic brain injury. [Methods] The 80 male SD rats were randomly divided into sham-operation group, model group, nimodiping group (positive control), and Compound Rhodiola high and low dose groups. Permanent models of middle cerebral artery occlusion by suture were made in rats. Drugs were given to the rats 7 d before establishment of the models, ig, once a day, for consecutive 10 d. The behavioral indexes were measured. The proportion of cerebral infarction was detected. Hippocampus tissue was separated, apoptosis rate of neurons was tested by using TUNEL method;the expression of Bcl-2 and Bax were observed by Western blot. [Results] Compared with sham-operation group, the scores of balance test of model group were increased, and grip strength was decreased and the proportion of cerebral infarction was increased (P<0.01). Apoptosis positive cells were evident increased. The expression of Bax was higher, but Bcl-2 was lower. Compared with model group, the scores of balance test of Compound Rhodiola high and low dose groups were decreased, and grip strength was increased and the proportion of cerebral infarction was decreased (P<0.01). Apoptosis positive cells were evident decreased. The expression of Bax was decreased, but Bcl-2 was increased. [Conclusion] The protection of Compound Rhodiola on ischemic brain injury through its inhibition of neuronal apoptosis, and the mechanism may be related with mitochondrial pathway.关键词
复方红景天/脑缺血/细胞凋亡/大鼠Key words
Compound Rhodiola/cerebral ischemia/apoptosis/rat分类
医药卫生引用本文复制引用
王宏,康利,刘庆焕,郝迪,王蕾,陶遵威..复方红景天对大鼠缺血性脑损伤后海马组织细胞凋亡的影响[J].天津中医药,2017,34(8):554-558,5.基金项目
天津市应用基础与前沿技术研究计划(一般项目) （一般项目）
天津市卫生和计划生育委员会科技基金项目(2015KR04). （2015KR04）
