新疆农业科学2017，Vol.54Issue(7)：1185-1190,6.DOI:10.6048/j.issn.1001-4330.2017.07.002
玉米ZmCIPK23基因Mutator插入突变体的鉴定
Identification of the Mutator Insertional Mutants in ZmCIPK23
摘要
Abstract
[Objective] The candidate mutants of maize ZmCIPK23 gene were continuously backcrossed and self-pollinated with B73 in fields, and finally homozygous mutants with B73 background were obtained, which laid the material foundation for studying the function of this gene.[Method]The candidate mutants of ZmCIPK23 were screened from a maize Mutant library.They were planted and analyzed by nested PCR.The positive plants with Mu-insertion in the ZmCIPK23 gene were self-pollinated and backcrossed with B73 in the field.Thus the homozygous mutants were obtained.[Result]The homozygous mutant of ZmCIPK23 gene was obtained by PCR.[Conclusion]The homozygous mutant (zmcipk23) of Mu insertion of ZmCIPK23 gene was obtained.This study has provided a foundation for the functional analysis of the ZmCIPK23 gene.关键词
玉米/Mutator转座子/ZmCIPK23/突变体Key words
maize (Zea Mays L.)/mutator transposon/ZmCIPK23/mutant分类
农业科技引用本文复制引用
陈果,陈勋基,李建平,郝晓燕,常晓春,足木热木,郑军,黄全生..玉米ZmCIPK23基因Mutator插入突变体的鉴定[J].新疆农业科学,2017,54(7):1185-1190,6.基金项目
新疆维吾尔自治区自然科学基金"玉米干旱胁迫相关基因Mu插入突变体的鉴定及抗旱性"(2013211B22)Xinjiang Natural Science Foundation " Identification and Drought Resistance Appraise of Mutants of Drought Stress-Related Genes in Maize" (2013211B22) （2013211B22）
