新疆农业科学2017，Vol.54Issue(7)：1208-1215,8.DOI:10.6048/j.issn.1001-4330.2017.07.005
两种油葵自交系再生体系的建立
Establishment of Regeneration System of Two Types Inbred Lines from Oil Sunflower (Helianthus annuus L.)
摘要
Abstract
[Objective] The explants of two inbred oil sunflower (1538-3 and 1573-2) were used to establish the tissue regeneration culture system, and the optimum strain was selected to lay the foundation for genetic transformation.[Method]Two different inbred lines of oil sunflower were taken respectively, and the regeneration of adventitious bud of oil sunflower was induced from two aspects of indirect and direct organs by different hormone ratio.[Result]The results showed that inbred lines 1538-3 was easy to form callus in the mediums containing NAA, 6-Benzylaminopurine (6-BA) and kinetin (KT), but all the callus were white with high water damage level.The sunflower cotyledonary node could induce adventitious buds when the concentration of 6-BA was 0 to 1.0 mg/L, which couldn't be observed in other hormone combinations.The combination of MS + 0.6 mg/L 6-BA + 0.1 mg/L GA3 was most suitable for the induction of adventitious bud from inbred lines 1573-2 hypocotyls explants.While for inbred line 1538-3, the most suitable medium was MS + 0.5 mg/L 6-BA + 0.05 mg/L GA3.The best concentration of NAA for inducing the root of adventitious buds ranged from 0 to 0.2 mg/L in both inbred lines.[Conclusion]The ability of the two genotypes of oil sunflower to induce adventitious bud is different.Among them, the inbred line 1538-3 has stronger regeneration ability, and it is better to choose the 1538-3 axis as the transformation receptor in the genetic transformation.关键词
油葵/胚轴/组织培养/再生体系Key words
Helianthus annuus L./hypocotyl/tissue culture/regeneration system分类
农业科技引用本文复制引用
周茜萍,王梦瑶,吕新华,祝建波,孙黎..两种油葵自交系再生体系的建立[J].新疆农业科学,2017,54(7):1208-1215,8.基金项目
国家自然科学基金项目"油葵DGAT1和DGAT2基因调控含油量和脂肪酸组分的分子机制"(31360052)the National Natural Science Foundation of China "Molecular Mechanism of DGAT1 and DGAT2 from Sunflower in Regulating Oil Content and Fatty Acid Composition" (31360052) （31360052）
