畜牧与兽医2017，Vol.49Issue(8)：16-20,5.
新疆细毛羊eIF3l基因的克隆、原核表达及蛋白检测
Cloning, prokaryoctic expression and protein measurement of the gene eIF3l in Xinjiang Fine-wool sheep
摘要
Abstract
In order to study the sequence characteristics,expression in E.coli BL21 (DE3) and identification of protein in vitro of eukaryotic translation initiation factor 3 L gene (eIF3l) in Xinjiang Fine-wool sheep,we cloned the CDS encoding sequence of eIF3l gene from the Xinjiang Fine-wool sheep fibroblasts by RT-PCR,and constructed the prokaryotic expression vector,induced the expression.The results showed that eIF3l gene sequences of CDS was got successfully,and the length of the CDS was 1 695 bp and coded 564 amino acids.The E.coli containing recombinant vector expressed inclusion body proteins of 69 ku after induction by 1 mmol/L IPTG.Western blotting revealed that the expressed protein was the target protein,which indicated that the recombinant prokaryotic expression vector expressed successfully.Together,our works made a foundation for the study of function and application of the eIF3l gene.关键词
新疆细毛羊/eIF3l基因/载体构建/原核表达Key words
Xinjiang Fine-wool sheep/eIF3l gene/vector construction/prokaryotic expression分类
农业科技引用本文复制引用
于常江,沈敏,杨华,杨永林,祁成年,张云生..新疆细毛羊eIF3l基因的克隆、原核表达及蛋白检测[J].畜牧与兽医,2017,49(8):16-20,5.基金项目
国家“863”计划(2013AA102506) （2013AA102506）
国家自然科学基金项目(30960249) （30960249）
兵团种质资源创新与功能基因发掘及利用专项(2012BB044) （2012BB044）
