畜牧与兽医2017，Vol.49Issue(8)：43-47,5.
梅花鹿胸腺素β4基因真核表达载体的构建及鉴定
Construction and identification of recombinant eukaryotic plasmid of sika deer thymosin beta4
摘要
Abstract
The present study was conducted to construct the eukaryotic expression vector of thymosin beta4 gene (Tβ4).The total RNA was extracted from sika deer antler by TRIzol regent and the coding region of the Tβ4 gene was amplified by RT-PCR,and then the gene fragment was inserted into the recombinant eukaryotic expression vector VR1012,and the eukaryotic expression plasmid was transfected into 293T cells by Fugene(R)6 agent.The expressed products were detected by Western blot and immunofluorescence.The ORF of Tβ34 cDNA was 135 bp,coding for a protein of 44 amino acids.Sika deer Tβ34 was cloned and constructed into VR1012.The expression of sika deer Tβ4 was detected in 293T cells by Western blot.Immunofluorescence detected the expressed fusion protein of the Tβ4-HA,which located in the cytoplasm in 293T cells.This finding lays experimental basis for study of Tβ4 roles in the antler growth.关键词
梅花鹿/胸腺素β4/质粒构建/转染/真核表达Key words
sika deer/thymosin beta4/plasmid construction/eukaryotic expression/transfection分类
农业科技引用本文复制引用
王佳雯,高云鹏,王敏,王思明,赵雨,白雪媛..梅花鹿胸腺素β4基因真核表达载体的构建及鉴定[J].畜牧与兽医,2017,49(8):43-47,5.基金项目
吉林省科技发展计划(20140622003JC) （20140622003JC）
