畜牧与兽医2017，Vol.49Issue(8)：76-80,5.
O型口蹄疫病毒不同宿主适应毒3A基因序列分析
Nucleotide and amino acid sequence analysis of 3A gene of foot and mouth disease virus type O in different hosts
摘要
Abstract
In order to analyze the nucleotide and amino acid sequences of the gene encoding 3A protein of Mya98 lineage foot and mouth disease virus (FMDV) type O adapted in different hosts and therefore viral evolution and variation of FMDV,FMDV strain O/X J/10-11 was inoculated in bovine,swine,rat and BHK-21 cells and the host-adapted FMDV strains were obtained.RNA in tissue fluid was extracted and used as the template for reverse transcription and amplification of 3A gene.Then these sequences were analyzed by Vector NTI 11.5 and DNAStar biological software.The results showed that in the four host-adapted virus strains from bovine,rat,swine and BHK-21 cells,3A gene was more stable,and variation occurred in aa 99 and aa 114,indicating that the two amino acids of FMDV 3A gene are related to host tropism to a certain extent.The results lay a foundation for further analysis of the variability of the genomes from the different host-adapted FMDV strains.关键词
口蹄疫病毒/O型/宿主适应毒/3A基因Key words
foot and mouth disease virus/type O/host-adapted virus/3A gene分类
农业科技引用本文复制引用
姚怀兵,赵毅,朱妍,张毅,刘宏,任方,黄炯..O型口蹄疫病毒不同宿主适应毒3A基因序列分析[J].畜牧与兽医,2017,49(8):76-80,5.基金项目
自治区科研机构创新发展专项资金(2016D04008) （2016D04008）
自治区产学研联合培养研究生示范基地项目(xjaucxy-yjs-20152008) （xjaucxy-yjs-20152008）
