畜牧与兽医2017，Vol.49Issue(8)：102-106,5.
猪传染性胃肠炎病毒LY86株N基因序列分析及病毒在猪睾丸细胞上的增殖特性研究
Sequence analysis of N gene of porcine transmissible gastroenteritis virus LY86 strain and its proliferation characteristics in swine testis cells
摘要
Abstract
This study aimed to investigate the differences in molecular biology between porcine transmissible gastroenteritis virus (TGEV) LY86 strain and other TGEV strains and its proliferation in swine testicular (ST) cells.The N gene of TGEV YL86 strain was amplified and sequenced,and ST cells were inoculated with TGEV YL86 strain at different multiplicity of infection (MOI) and virus titers were measured at different time points post infection.The results showed that the N gene of TGEV YL86 strain shared 97.2％ to 99.8％ homology with TGEV reference strains,and there was a high homology between TGEV LY86 strain and M6,M60,TS,H,H16 or JS2012 strains.MOI had an important effect on proliferation of TGEV YL86 strain in ST cells.The virus titer reached peak of 10620±0.23TCID50/0.1 mL at 48 hours post infection with an MOI of 0.001.These results have great significance in analyzing the genetic variation and infectious characteristics of TGEV and optimizing vaccine production process.关键词
猪传染性胃肠炎病毒/猪睾丸细胞/N基因/序列分析/增殖特性Key words
transmissible gastroenteritis virus of swine/ST cells/N gene/sequence analysis/proliferation characteristics分类
农业科技引用本文复制引用
杨元奇,季艳杰,张金秋,苗晋锋..猪传染性胃肠炎病毒LY86株N基因序列分析及病毒在猪睾丸细胞上的增殖特性研究[J].畜牧与兽医,2017,49(8):102-106,5.基金项目
国家自然科学基金项目(31672515) （31672515）
