中国康复理论与实践2017，Vol.23Issue(8)：905-911,7.DOI:10.3969/j.issn.1006-9771.2017.08.008
沉默ski基因对大鼠星形胶质细胞迁移的影响
Effect of Knocking Down ski on Migration of Astrocytes in Rats
摘要
Abstract
Objective To investigate the effect of ski gene in migration process of astrocytes in rats. Methods Astrocytes were obtained from rats' cerebral cortex and cultured in vitro. siRNA targeting ski gene and negative control sequences were prepared. The ski-siRNA group, siRNA negative control group and untreated group were set in this experiment. The specific siRNA targeting ski gene was transfected into astrocytes with Lipofectamine?RNAiMAX Reagent. Then the ski protein levels were determined with Western blotting. After transfec-tion, the changes in migration of astrocytes were measured with wound scratch assay and Transwell migration assay. Results Western blot-ting showed that the expression of ski protein was significantly lower in the ski-siRNA group than in the siRNA negative control group and untreated group (F=132.957, P<0.001). Transwell migration assay showed that the number of astrocytes crossing through chambers was less in the ski-siRNA group than in the siRNA negative control group and untreated group (F>47.197, P<0.05). Wound scratch assay showed that the wound healing rate was lower in the ski-siRNA group than in the control group one, two, three, four and five days after transfection (F>69.187, P<0.001). Conclusion Ski knocked down by siRNA could inhibit the migration ability of astrocytes. It is a reminding that ski may take part in the migration process of astrocytes, and moreover, ski may play an important role in the formation of glial scar.关键词
ski/RNA干扰/星形胶质细胞/Transwell细胞迁移实验/划痕实验/大鼠Key words
ski/siRNA/astrocyte/Transwell migration assay/wound scratch assay/rats分类
医药卫生引用本文复制引用
赵鑫,王兴文,寇江力,李忠浩,郭永强,伍亚民,张海鸿..沉默ski基因对大鼠星形胶质细胞迁移的影响[J].中国康复理论与实践,2017,23(8):905-911,7.基金项目
1.国家自然科学基金项目(No.30772299) （No.30772299）
2.兰州大学第二医院科研基金项目(No.sdkyjj-04). （No.sdkyjj-04）
