摘要
Abstract
Objective To study species and constituent ratio of pathogen of 200 cases of patients with sepsis and to study drug resistance rate of major bacteria via drug resistance analysis,so as to provide theory gist for clinical diagnosis and treatment of sepsis.Methods 200 cases of patients with sepsis treated in People′s Hospital of Wuhan University since February 2014 to February 2017 were selected as the object of study.All patients were collected for blood samples which were separately cultured for bacteria via BD9120 automatically blood culture instrument and were observed for species and constituent ratio and other distributions of pathogen.Drug resistance analysis was conducted via American BD Compact automatically bacteria identification and drug sensitivity analysis system and were respectively detected for drug resistance of gram-positive bacterium and gram negative bacterium and other major bacteria against antimicrobial.Results Among 200 cases of patients with sepsis,there were 220 strains of pathogens detected in blood samples,in which the ratio of gram negative bacterium was greatest with 143 strains and a proportion for 65.00%.The top four of constituent ratio included klebsiella pneumoniae,escherichia coli,pseudomonas aeruginosa and cloaca entero bacter whose proportion were 18.18%(40/220),17.27%(38/220),6.36%(14/220),5.45%(12/220)respectively.There were 65 strains of gram positive bacteria with proportion for 29.55%,and the top three of constituent ratio included coagulase-negative staphylococci,staphylococcus aureus and enterococcus with proportion for 17.73%(39/220),5.45%(12/220),4.55%(10/220)respectively.There were 12 strains of funguses with proportion for 5.45%.The detection rate of escherichia coli spectrum β-lactamase producing in gram negative bacterium was 68.42%(26/38),and that of Klebsiella pneumoniae was 22.50%(9/40).The proportion of drug resistance strains of methicillin resistant staphylococcus aureus in gram positive bacterium was 25.00%(3/12),and that of coagulase-negative staphylococci was 82.05%(32/39).The drug resistance of gram negative bacterium and gram positive bacterium and other major bacteria in blood of patients with sepsis against antimicrobial was different,and the sensitivity over imipenem and vancomycin was the highest.Conclusion The pathogens of patients with sepsis are mainly constituted with gram negative bacterium,and then gram positive bacterium with small proportion of fungal infection.The result of drug resistance analysis is not optimistic and there are different degrees of drug resistance against conventional antimicrobial.Antibiotic shall be applied in accordance with pathogen distribution and drug resistance for treatment in clinic and the abuse of antibiotic shall be avoid.关键词
出血性败血症,病毒性/病原菌分布特征/耐药性分析Key words
Hemorrhagic septicemia,viral/Pathogen distribution,Drug resistance analysis分类
医药卫生
