中国全科医学2017，Vol.20Issue(z1)：81-83,3.
C3AR1基因对K562细胞增殖及侵袭影响的机制研究
Effect of C3AR1 Gene on the Proliferation and Invasion of K562 Cells
李娟 1封忠昕1
作者信息
- 1. 563000 贵州省遵义市,遵义医学院附属医院
- 折叠
摘要
Abstract
Objective To investigate the effect of C3AR1 gene on the proliferation and invasion of chronic myeloid leukemia cell line K562 and its mechanism.Methods Construction of HL-60-C3AR1 overexpressing K562 cell line and its expression.The expression of C3AR1 mRNA and protein was detected by RT-PCR and Western blotting.The proliferation of K562 cells was detected by CCK8 method.The invasive ability was evaluated by trans well assay,and the possible mechanism was analyzed by PCR chip expression.Results The expression of C3AR1 mRNA and C3AR1 protein in K562-C3AR1 were significantly higher than those in K562 and K562-Scramble groups(P<0.05).The cell proliferation and cell invasion of C3AR1 were significantly higher than those of K562 and K562-Scramble(P<0.05).PCR chip analysis showed that the expression of 7 genes was increased and the down-regulation of 4 genes could be involved in the proliferation and invasion of K562-C3AR1.Conclusion C3AR1 gene plays an important role in regulating K562 cells.Overexpression of C3AR1 gene can promote the proliferation and invasion of K562 cells.The specific mechanism may be related to DBH and EPS8 genes.关键词
白血病,髓样/C3AR1基因/细胞增殖/肿瘤侵润Key words
Leukemia,myeloid/C3AR1 gene/Cell proliferation/Neoplasm invasiveness分类
医药卫生引用本文复制引用
李娟,封忠昕..C3AR1基因对K562细胞增殖及侵袭影响的机制研究[J].中国全科医学,2017,20(z1):81-83,3.
