中国畜牧兽医2017，Vol.44Issue(8)：2215-2225,11.DOI:10.16431/j.cnki.1671-7236.2017.08.001
京海黄鸡G蛋白偶联受体1基因生物信息学及组织表达分析
Bioinformatics and Tissue Expression Analysis of G Protein-coupled Receptor 1 Gene in Jinghai Yellow Chicken
摘要
Abstract
This study was aimed to investigate the possible genes that involved in hormone regulation and regulate ovarian function,and selected G protein-coupled receptor 1 to in-depth study whether it was involved in hormone regulation.In this study,a pair of primers was designed based on the CDS sequence of GPR1 gene of Jinghai Yellow chicken which according to transcriptome sequencing.Real-time RT-PCR was used to detect the expression of GPR1 gene in the ovary of high and low-production Jinghai Yellow chicken.Bioinformatics analysis of GPR1 gene was performed by using combined biological softwares and online tools.The homology of GPR1 gene with other species,the physicochemical properties of protein,the transmembrane region,subcellular localization,hydrophilicity,potential phosphorylation site,conserved domain of protein sequence,secondary structure and tertiary structure of GPR1 protein were analyzed.The results showed that GPR1 gene of Jinghai Yellow chicken shared 100.0％,69.0％,69.1％,69.3％,68.9％,69.6％,69.2％,68.8％,68.9％,67.5％,69.0％and 50.3％identity with Gallus gallus,Homo sapiens,Bos taurus,Sus scrofa,Pan troglodytes,Leporidae,Equus caballus,Ovis aries,Pantholops hodgsonii,Rattus norvegicus,Mus musculus and Danio rerio,respectively.GPR1 protein structure showed that the molecular weight was 40.41 ku and pI was 6.91,including seven transmembrane segments which were consistent with the transmembrane analysis.Subcellular localization showed that GPR1 protein belonged to non-secretory protein.The predicted results showed that the protein contained 25 potential phosphorylation sites and 1 glycosylation sites.The secondary structure of GPR1 protein was mainly composed of random coil.The tertiary structure of domain area of GPR1 protein showed a transmembrane helix and single strand structure.The result of tissue expression showed that the expression of GPR1 gene in the low-production group was extremely significantly higher than the high-production group(P<0.01).The results of the study would be beneficial to the further analysis of the function of GPR1 gene,and provided a theoretical basis for the further study of the gene on the regulation mechanism of laying traits of Jinghai Yellow chicken.关键词
京海黄鸡/G蛋白偶联受体1/生物信息学分析/蛋白/组织表达Key words
Jinghai Yellow chicken/G protein-coupled receptor 1/bioinformatics analysis/protein/tissue expression分类
农业科技引用本文复制引用
袁曼曼,张涛,张向前,李婷婷,凌娇娇,吴鹏飞,王金玉,施会强..京海黄鸡G蛋白偶联受体1基因生物信息学及组织表达分析[J].中国畜牧兽医,2017,44(8):2215-2225,11.基金项目
国家肉鸡产业技术体系(nycytx-42-G1-05) （nycytx-42-G1-05）
江苏高校优势学科建设工程 （）
江苏省动物遗传繁育与分子设计重点实验室 （）
