中国畜牧兽医2017，Vol.44Issue(8)：2241-2247,7.DOI:10.16431/j.cnki.1671-7236.2017.08.004
基因编辑技术及其应用的研究进展
Research Advances on Techniques of Gene Editing and Its Application
摘要
Abstract
Gene editing techniques(GETs)can introduce mutations precisely into endogenous genome at a designed location.GETs are widely used in the research of bio-sciences,and promote a great development in life sciences.So far,most of the artificial gene mutations are generated by the following three GETs,including zinc finger nucleases(ZFN),transcription activator-like effector nucleases(TALEN)and clustered regularly interspaced short palindromic repeats/Cas endonuclease(CRISPR/Cas).GETs can recognize and modify the target sequence of endogenous gene with specificity,which are good ways to study the function of new genes.GETs have the advantage of simple operation,high efficiency and accuracy,so it is widely used in life science research,constraction of disease models,drug research and development and agricultural production.This review presents the three different nuclease-based GETs,including the mechanisms by which they produce genetic modifications,their recent advances and prospects as well as the differences between GETs and transgene techniques.关键词
基因编辑/锌指核酸酶/类转录激活因子效应物核酸酶/规律性重复短回文序列簇Key words
gene editing/ZFN/TALEN/CRISPR/Cas分类
生物科学引用本文复制引用
李想,崔文涛,李奎..基因编辑技术及其应用的研究进展[J].中国畜牧兽医,2017,44(8):2241-2247,7.基金项目
国家转基因生物重大专项重大课题"节粮型高瘦肉率转基因猪新品种培育"(2016ZX08006-001) （2016ZX08006-001）
