中国畜牧兽医2017，Vol.44Issue(8)：2248-2254,7.DOI:10.16431/j.cnki.1671-7236.2017.08.005
东北黑熊源粪肠球菌EfaA基因的克隆表达及生物信息学分析
Cloning, Expression and Bioinformatics Analysis of Enterococcus faecalis from Northeast Black Bear
摘要
Abstract
In order to detect Enterococcus faecalis endocarditis antigen(EfaA)of bear that was used to disclose the infected Northeast Black bear immediately,a pair of PCR primers was synthesized by EfaA gene of Enterococcus faecalis that was recorded in GenBank(accession number: U03756.1)and the target fragment was 689 bp.The PCR product was cloned by pMD19-T vector,then was transferred to Escherichia coli DH5α competent cells and the positive clones were filtered.Recombinant plasmid(pMD19-T-EfaA)was extracted.Identification of PCR and digestion,sequencing,the structure prediction of proteins were operated.The results showed that EfaA gene was cloned successfully,and the gene homology with ATCC 29212 was 100.0％.pMD19-T-EfaA was constructed successfully,structure of proteins was predicted.This study provided theoretical basis for diagnostic methods of Enterococcus faecalis and laid basis for prevention and control of Northeast Black bear disease.关键词
东北黑熊/粪肠球菌心内膜炎抗原(EfaA)/基因克隆/生物信息学分析Key words
Northeast Black bear/Enterococcus faecalis endocarditis antigen (EfaA)/gene cloning/bioinformatics analysis分类
农业科技引用本文复制引用
秦晓东,楚熹橦,鲁承,孙福亮,孙兴忠..东北黑熊源粪肠球菌EfaA基因的克隆表达及生物信息学分析[J].中国畜牧兽医,2017,44(8):2248-2254,7.基金项目
延边大学青年基金项目(602016032) （602016032）
