中国畜牧兽医2017，Vol.44Issue(8)：2269-2276,8.DOI:10.16431/j.cnki.1671-7236.2017.08.008
小鼠急性肺损伤造模条件的探究
Study on the Building Conditions of Acute Lung Injury in Mice
摘要
Abstract
In order to explore the building conditions of acute lung injury,healthy Kunming mice were selected,by intraperitoneal injection of cascade dose of LPS(0,2,4,6,8,10 mg/kg)to observe the mice respiratory frequency,mortality,lung W/D value and the changes of tissue structure in different conditions,and detect the expression changes of inflammation cytokine,IL-10,TNF-α,IL-1β and IFN-γ.The results showed that compared with control group,lung W/D value in 4 mg/kg LPS group significantly increased(P<0.05)and lung W/D values in 6,8 and 10 mg/kg LPS groups extremely significantly increased(P<0.01).Pathological analysis showed that 6 mg/kg LPS group appeared lung congestion,alveolar luminal and pulmonary interstitial exudation,alveolar septal thickening,and a small amount of neutrophil infiltration.8 and 10 mg/kg LPS groups appeared pulmonary congestion,alveolar and pulmonary interstitial exudation,alveolar septal thickening and neutrophil infiltration.Compared with control group,collagen fibers of the lung tissue in 6,8,10 mg/kg LPS group increased,and more appeared in the lung around the trachea,10 mg/kg LPS group was the most obvious.The inflammatory factors in 2,4,6,8 and 10 mg/kg LPS groups had extremely significantly increased compared with control group(P<0.01).Pathological biopsy and related indicators showed that the model of mice could be successfully constructed by intraperitoneal injection of 8 mg/kg LPS for 12 h.关键词
急性肺损伤/小鼠/脂多糖/肺组织Key words
acute lung injury/mouse/lipopolysaccharide (LPS)/lung tissue分类
农业科技引用本文复制引用
罗友,吴欣瞳,庞欣欣,肖珂,彭克美,宋卉..小鼠急性肺损伤造模条件的探究[J].中国畜牧兽医,2017,44(8):2269-2276,8.基金项目
国家自然科学基金青年基金项目(31101776) （31101776）
华中农业大学自主科技创新项目(2662015PY063) （2662015PY063）
