中国畜牧兽医2017，Vol.44Issue(8)：2283-2288,6.DOI:10.16431/j.cnki.1671-7236.2017.08.010
核苷二磷酸激酶B稳定表达细胞系的建立及其对新城疫病毒F48E9株复制的影响
Establishment of a Stable Expression Cell Line of Nucleoside Diphosphate Kinase B and Its Influence on Replication of F48E9 Strain of Newcastle Disease Virus
摘要
Abstract
In order to explore the influence of nucleoside diphosphate kinase B(NDPK B)on replication and proliferation of F48E9 strain of Newcastle disease virus(NDV),the eukaryotic expression plasmid pEGFP-NDPK B was transfected into Vero cells,then we got the cell subclones by G418 screening.We identified the expression of NDPK B protein by RT-PCR,Western blotting and inverted flurescence microscope.On the basis of Vero/NDPK B cell line,we explored the influence of NDPK B on replication and proliferation of F48E9 strain of NDV by HA-HI,TCID50 and Real-time PCR.The results showed that we had successfully constructed the cell line named Vero/NDPK B,which could stably express NDPK B protein,and found that NDPK B could inhibit the replication and proliferation of F48E9 strain of NDV in Vero cells.It suggested that on the basis of NDPK B,we could design and develop new drugs or antiviral synergist to prevent or treat NDV.关键词
核苷二磷酸激酶B/表达/新城疫病毒/复制与增殖Key words
nucleoside diphosphate kinase B (NDPK B)/expression/Newcastle disease virus (NDV)/replication and proliferation分类
农业科技引用本文复制引用
郭育培,班付国,闫若潜,方先珍,丁壮..核苷二磷酸激酶B稳定表达细胞系的建立及其对新城疫病毒F48E9株复制的影响[J].中国畜牧兽医,2017,44(8):2283-2288,6.基金项目
河南省科技创新人才计划项目(豫科人组[2011]1号) （豫科人组[2011]1号）
