中国畜牧兽医2017，Vol.44Issue(8)：2319-2325,7.DOI:10.16431/j.cnki.1671-7236.2017.08.015
饲用产甘露聚糖酶芽孢杆菌的筛选、鉴定及其对肉鹅生长性能影响的研究
Study on Screening and Identification of Bacillus Producing Mannase and Its Effect on Growth Performance of Geese
摘要
Abstract
The aim of this study was to select a strain of producing mannase Bacillus,and investigate its effects on growth performance of geese.Congo red plate changing color circle method and assay of enzyme activity method in liquid fermentation were used in screen and rescreen,respectively.Animal safety and gastrointestinal tolerability tests were alse carried out.By morphological observation,physiological and biochemical tests,analysis of complete sequence of 16S rDNA and the phylogenetic tree establishment,species were identified.Bacillus were prepared and added by 0.10％,0.25％,0.50％,0.75％and 1.00％in the geese diets to study its effect on the daily gain and F/G in geese.The results showed that the enzyme activity of the isolated Bacillus subtilis Y-3-2 was 45.939 U/mL,and the strain could also endure pH 2.0 and grow normally in 0.1％cholic acid salt solution and artificial intestinal fluid.The results of feeding test showed that,compared to the control group,the average daily gain of geese increased by 8.78％(P<0.01)and F/G decreased by 6.69％(P<0.01)by adding 0.25％Y-3-2 preparation.In conclusion,adding mannase producing Bacillus could improve the feed utilization and growth of geese.关键词
芽孢杆菌/甘露聚糖酶/胃肠道耐受性/肉鹅/生长性能Key words
Bacillus/mannase/gastrointestinal tolerability/goose/growth performance分类
农业科技引用本文复制引用
张学雯,李红亚,齐聪岩,王树香,王全,李术娜..饲用产甘露聚糖酶芽孢杆菌的筛选、鉴定及其对肉鹅生长性能影响的研究[J].中国畜牧兽医,2017,44(8):2319-2325,7.基金项目
河北省教育厅科学技术研究项目(2011232) （2011232）
