中国畜牧兽医2017，Vol.44Issue(8)：2431-2436,6.DOI:10.16431/j.cnki.1671-7236.2017.08.030
罗伊氏乳杆菌抑制致病性大肠杆菌感染效果的研究
Effects of Lactobacillus reuteri Supplementation on Inhibition of Pathogenic Escherichia coli in Kunming Mice
摘要
Abstract
This study was aimed to explore the effect,dose of Lactobacillus reuteri on inhibition of pathogenic Escherichia coli infection on Kunming mice as well as its potential mechanism.96 Kunming mice were randomly allocated into four groups with 4 replicates per group and 6 mice per replicate,which were control group,low dose group,middle dose group and high dose group.All of the mice feed commercial basal feedstuff,meanwhile the mice in control group drank tap-water,three treatments were supplemented with 1.0×106,1.0×107 and 1.0×108 CFU/mL Lactobacillus reuteri in water.The feeding period was 28 days.All the mice were weighted,and the ADFI,ADG and F/G were calculated at the end of experiment.At the day of 28,pathogenic Escherichia coli(4.98×109 CFU/mL)were irrigated to study the mortality.The indexes of intestinotoxin and antioxidation in serum and cecal microbacteria were inspected.The results indicated that supplemented with middle dose of Lactobacillus reuteri could significantly improve the final body weight,ADG compared with the control(P<0.05).The mortality,serum intestinotoxin level and MDA concentration,Escherichia coli and Staphylococcus were significantly lower than control group(P<0.05),while the T-SOD,T-AOC,GSH-Px,Lacobacillus and Bifidobacterium were significantly improved(P<0.05).In conclusion,with Lactobacillus reuteri supplementation,the growth performance was improved and antioxidant capacity and cecal microbacteria were enhanced after pathogenic Escherichia coli infected,mouse mortality caused by the pathogenic Escherichia coli was effectively reduced,and the effect of 1.0×107 CFU/mL Lactobacillus reuteri was the best.关键词
罗伊氏乳杆菌/昆明鼠/致病性大肠杆菌/抗氧化力Key words
Lactobacillus reuteri/Kunming mice/pathogenic Escherichia coli/antioxidant capacity分类
农业科技引用本文复制引用
杨家军,钱坤,李庆岗,吴义景,吴东,王重龙..罗伊氏乳杆菌抑制致病性大肠杆菌感染效果的研究[J].中国畜牧兽医,2017,44(8):2431-2436,6.基金项目
安徽省农业科学院学科建设项目(16A0410) （16A0410）
安徽省生猪现代农业项目(皖农科2013-2016) （皖农科2013-2016）
安徽省农业科学院博士启动项目(皖农科2012) （皖农科2012）
