中国畜牧杂志2017，Vol.53Issue(8)：34-37,43,5.DOI:10.19556/j.0258-7033.2017-08-034
猪ZBED6基因有效突变位点的筛选与验证
Screening and Identification of Effective Mutation Site in Pig ZBED6 Gene
摘要
Abstract
The mutation of ZEBD6 can promote the transcription of IGF2 in skeletal muscle,which could enhance the cell proliferation and myotube formation.In this study,CRISPR-Cas9 was used to editing the pig ZEBD6 gene.Four mutation targets were screened by online software,pCas9/gRNA-ZBED6 plasmid was constructed by pCas9/gRNA vector.At the same time,pTYNE-ZBED6 plasmid was constructed to verify the mutation efficient.These plasmids were transfected to HEK293 cell line in the meantime.The fluorescence signal was observed and was used to judge the mutation efficient of the target sequence.The results showed that,the f target 1,which is located in 17-37 nucleotide acid,has the strongest fluorescence signal.It means that CRISPR-Cas9 has the highest cutting efficiency in this site.It is the effective mutation site in ZBED6 gene which can be edited by CRISPR-Cas9.关键词
猪/ZBED6基因/荧光信号/CRISPR-Cas9技术Key words
Pig/ZBED6 gene/Fluorescence signal/CRISPR-Cas9 technology分类
农业科技引用本文复制引用
张冬杰,汪亮,李忠秋,刘娣..猪ZBED6基因有效突变位点的筛选与验证[J].中国畜牧杂志,2017,53(8):34-37,43,5.基金项目
农业部畜禽遗传资源与种质创新重点实验室开放课题 （）
国家生猪产业技术体系(CARS-36) （CARS-36）
