中国药理学通报2017，Vol.33Issue(9)：1211-1214,4.DOI:10.3969/j.issn.1001-1978.2017.09.007
尿酸酶-过氧化氢酶脂质体的特性分析及药效学研究
Property and pharmacodynamics of uricase-catalase liposomes
摘要
Abstract
Aim To observe the optimal temperature and optimal pH of uricase-catalase liposomes(UCALP) and free uricase(UAE), and study the abilities of UCALP to reduce uric acid and hydrogen peroxide in mice with hyperuricemia.Methods UCALP were prepared by reverse phase evaporation, optimal temperature and optimal pH of UCALP and UAE were determined, respectively.Mouse model of hyperuricemia was established by intraperitoneally injection of uric acid, and the model mice were intravenously injected UCALP and UAE, respectively, then the serum concentration of uric acid and hydrogen peroxide in mice at different time points were measured by the assay kits, respectively.Results Optimal temperature of UCALP and UAE was 40℃, and optimal pH was 8.0 and 8.5, respectively.UCALP could more significantly lower uric acid level of hyperuricemia mice than that of UAE, and the concentration of hydrogen peroxide in UCALP group was lower than in UAE group.Conclusion UCALP can effectively decrease the level of uric acid and control the level of hydrogen peroxide in mice with hyperuricemia.关键词
尿酸酶/过氧化氢酶/脂质体/高尿酸血症/小鼠/药效学/最适温度和最适pHKey words
uricase/catalase/liposome/hyperuricemia/mice/pharmacodynamics/optimal temperature and optimal pH分类
医药卫生引用本文复制引用
杨兰,周云莉,李瑶,晏声蕾,张景勍..尿酸酶-过氧化氢酶脂质体的特性分析及药效学研究[J].中国药理学通报,2017,33(9):1211-1214,4.基金项目
国家自然科学基金资助项目(No 30973645) （No 30973645）
重庆市研究生科研创新项目资助(No CYS 16133) （No CYS 16133）
