中国药理学通报2017，Vol.33Issue(9)：1235-1242,8.DOI:10.3969/j.issn.1001-1978.2017.09.011
基于核磁共振的益母草碱抗急性心肌缺血的代谢组学研究
1H NMR-based metabolomics study of intervention effects ofleonurine on acute myocardial ischemia in rats
摘要
Abstract
Aim To investigate metabolomic profiles of acute myocardial ischemia (AMI) in rat plasma and explore the intervention effects and its mechanism of leonurine using a metabolomics approach based on nuclear magnetic resonance (NMR).Methods The plasma metabolomic characteristics in rats of sham group,AMI model group,and leonurine-treated group were detected by 1H NMR,and the different metabolites between AMI group and sham group or leonurine-treated group were analyzed by pattern recognition and multivariate data analysis.Results Orthogonal partial least squares discriminant analysis (OPLS-DA) demonstrated that six metabolites related to AMI were screened out,including alanine,lysine,glycine,creatine,N-acetyl glycoprotein,and O-acetyl glycoprotein and all their levels were elevated in AMI group compared to sham group.Treatment of leonurine decreased the levels of alanine,lysine,and glycine,and increased the levels of choline,phosphocholine,and scyllo-inositol compared with the model group.Conclusions Leonurine can improve amino acid metabolism disorder under AMI conditions and enhance the function of choline and inositol pathway,which may explain its cardioprotective effect.The developed metabolomics approach in this study is a powerful tool for the investigation of the cardioprotective effect of leonurine and provide a new insight to understand its pharmacological mechanism.关键词
益母草碱/急性心肌缺血/核磁共振/代谢组学/模式识别/多变量统计Key words
leonurine/acute myocardial ischemia/NMR/metabolomics/pattern recognition/multivariate statistics分类
医药卫生引用本文复制引用
刘成鹏,殷晓芹,李玉琴,罗珊珊,朱清,李俊旭,朱依谆..基于核磁共振的益母草碱抗急性心肌缺血的代谢组学研究[J].中国药理学通报,2017,33(9):1235-1242,8.基金项目
国家自然科学基金资助项目(No 81373390) （No 81373390）
国家重点基础研究发展计划资助项目(No 2010CB912600) （No 2010CB912600）
江苏省自然科学基金资助项目(No BK20130396) （No BK20130396）
