中国药理学通报2017，Vol.33Issue(9)：1253-1259,7.DOI:10.3969/j.issn.1001-1978.2017.09.014
p38 MAPK反义寡聚脱氧核苷酸抑制脑缺血预处理诱导脑缺血耐受过程中GLT-1表达上调
p38 MAPK antisense oligodeoxynucleotide inhibited up-regulation ofGLT-1 during induction of brain ischemic tolerance induced bycerebral ischemic preconditioning
摘要
Abstract
Aim To investigate the effect of p38 MAPK AS-ODNs on the expression of GLT-1 during the induction of brain ischemic tolerance induced by cerebral ischemic preconditioning (CIP).Methods Eighty healthy male Wistar rats with permanent occlusion of the bilateral vertebral arteries were randomly divided into 6 groups: ①Sham group (n=10);②CIP group (n=10);③ischemic insult (Ⅱ) group (n=10);④CIP+Ⅱ group (n=10);⑤p38 MAPK AS-ODNs+CIP+Ⅱ group (n=30);⑥p38 MAPK S-ODNs+CIP+Ⅱ group (n=10).Group ⑤ was divided into 5 nmol, 10 nmol and 15 nmol subgroups according to the dose of p38 MAPK AS-ODNs (n=10).The dose of p38 MAPK S-ODNs was 15 nmol.All the rats were sacrificed 6 h and 2 d after the sham operation or the last time of global brain ischemia reperfusion.Western blot and immunohistochemistry analysis were used for detecting the expression of p-p38 MAPK and GLT-1 protein.Results CIP moderately up-regulated the expression of p-p38 MAPK and significantly up-regulated the expression of GLT-1 protein, inhibited the excessively up-regulation of p-p38 MAPK and the down-regulation of GLT-1 induced by ischemic insult.p38 MAPK AS-ODNs significantly inhibited the up-regulation of p-p38 MAPK and GLT-1 protein in a dose-dependent manner during the induction of brain ischemic tolerance by CIP.Conclusion p38 MAPK AS-ODNs inhibit the up-regulation of GLT-1 during the induction of brain ischemic tolerance induced by CIP.关键词
p38MAPK/GLT-1/AS-ODNs/脑缺血预处理/海马/大鼠Key words
p38 MAPK/GLT-1/AS-ODNs/cerebral ischemic preconditioning/hippocampus/rat分类
医药卫生引用本文复制引用
王佳磊,蒋孟洋,张孟岳,王文帅,李力,张玲燕,李文斌,张敏..p38 MAPK反义寡聚脱氧核苷酸抑制脑缺血预处理诱导脑缺血耐受过程中GLT-1表达上调[J].中国药理学通报,2017,33(9):1253-1259,7.基金项目
国家自然科学基金资助项目(No 31271149,81271454) （No 31271149,81271454）
河北省自然科学基金资助项目(No H2015206492) （No H2015206492）
河北省人才培养工程资助项目(No A201400544) （No A201400544）
