中国药理学通报2017，Vol.33Issue(9)：1291-1297,7.DOI:10.3969/j.issn.1001-1978.2017.09.020
基于促甲状腺功能的姜黄素延长低温冷冻小鼠生存时间的作用机制研究
Effect of curcumin in prolonging survival timeof cryopreserved mice via promoting thyroid function
摘要
Abstract
Aim To look for cold-protective drugs treating cryogenic freezing that may bring great damage to animal physiological system.Methods The protective effect of curcumin on frozen damage and the changes of thyroid function in mice were studied in this study.Quantitative analysis of the changes in survival time and metabolic indexes in mice disposed at(-20±1)℃ was conducted.Mouse serum free triiodothyronine(FT3) and free thyroxine(FT4) contents were detected by ELISA kit.HE staining was used to observe thyroid tissue morphological items.The expression of genes related to thyroid function was assessed via real-time quantitative PCR.Results The intraperitoneal injection of curcumin(12.5~50 mg·kg-1) could remarkably prolong the survival time of mice when exposed to cryogenic freezing.HE staining results displayed a recovered thyroid injury in morphology in the curcumin group, further with a notably improved metabolic indexes and evidently increase in serum FT3 and FT4 levels.The real-time quantitative PCR results indicated that the expressions of sodium iodide symporter(Nis), thyroglobulin(Tg) and thyroid peroxidase(Tpo) were up-regulated, and the expression of thyroid-stimulating hormone receptor(Tshr) was down-regulated.Sodium levothyroxine collabrated with the promoting thyroid effects of curcumin, while propylthiouracil inhibited the effects.Conclusion Curcumin can prolong the survival time of the cryogenic freezing mice, which is closely related to its ability to promote thyroid function.关键词
姜黄素/冷冻损伤/甲状腺/生存时间/昆明小鼠/丙硫氧嘧啶/左旋甲状腺素钠Key words
curcumin/cold injury/thyroid/survival time/Kunming mice/propylthiouracil/sodium levothyroxine分类
医药卫生引用本文复制引用
李照雪,胡思远,王吉烨,陈泽慧,张闪闪,张波..基于促甲状腺功能的姜黄素延长低温冷冻小鼠生存时间的作用机制研究[J].中国药理学通报,2017,33(9):1291-1297,7.基金项目
国家自然科学基金资助项目(No 81460566,U1603122) （No 81460566,U1603122）
