中国药理学通报2017，Vol.33Issue(9)：1303-1308,6.DOI:10.3969/j.issn.1001-1978.2017.09.022
杠板归总黄酮抗大鼠肝纤维化作用的机制研究
Research on mechanism of effect of total flavonoidsextracted from Polygonum perfoliatum L.onanti-hepatic fibrosis in rats
摘要
Abstract
Aim Based on the effect of total flavonoids extracted from Polygonum perfoliatum L.(TFP) against dimethylnitrosamine(DMN)-induced hepatic fibrosis(HF), to investigate the anti-fibrotic mechanism of TFP.Methods Ninety SD rats were divided into normal group, model group, colchicines(0.1 mg·kg-1) group, and TFP(200, 100, 50 mg·kg-1) group.Except the rats of normal group, other rats were injected intraperitoneally with volume fraction 0.5% DMN solution(2 mL·kg-1) for eight weeks, once every two days.From the first day of modeling, each administration group was given the corresponding dose of drugs to intervene, and the normal group and model group were given an equal volume of solvent, once a day.At the end of the eighth week, the blood and liver tissues were collected.Liver tissue was taken at a fixed position, and the degree of liver tissue was observed by HE staining.The contents of serum ALT, AST, SOD and MDA were measured using colorimetric method;the levels of serum HA, LN, PCⅢand Ⅳ-C were detected using enzyme-linked immunosorbent assay(ELISA);the levels of TNF-α, IL-1β and IL-6 in liver tissues were detected by ELISA;the expression of α-SMA, TGF-β1, p-JAK2 and p-STAT3 were detected by Western blot.Results Compared with the model group, TFP(200, 100, 50 mg·kg-1) could improve the liver tissue lesions, reduce the expression of ALT, AST, HA, LN, PCⅢ, IV-C and MDA, increase SOD activity, reduce the levels of TNF-α, IL-1β and IL-6, and inhibit the expression of α-SMA, TGF-β1, JAK2, STAT3, p-JAK2 and p-STAT3.Conclusion TFP could inhibit DMN-induced HF of rats, which may be involved with antioxidant and inhibiting expression of TGF-β1, JAK2/STAT3 signaling pathway and inflammatory response.关键词
杠板归总黄酮/肝纤维化/二甲基亚硝胺/抗氧化/TGF-β1/JAK2/STAT3信号通路/炎症反应Key words
total flavonoids extracted from Polygonum perfoliatum L.(TFP)/hepatic fibrosis/dimethylnitrosamine/antioxidant/transforming growth factor-β1/JAK2/STAT3 signaling pathway/inflammatory response引用本文复制引用
曹后康,高雅,黄思茂,张可锋..杠板归总黄酮抗大鼠肝纤维化作用的机制研究[J].中国药理学通报,2017,33(9):1303-1308,6.基金项目
国家自然科学基金资助项目(No 81460602) （No 81460602）
