中国药理学通报2017，Vol.33Issue(9)：1309-1314,6.DOI:10.3969/j.issn.1001-1978.2017.09.023
白藜芦醇抑制人肝癌SMMC-7721细胞增殖并降低mTOR蛋白磷酸化水平
Effect of resveratrol on proliferation of liver cancer SMMC-7721 cells andlowering levels of mTOR protein phosphorylation
摘要
Abstract
Aim To study the effect of resveratrol on proliferation of liver cancer SMMC-7721 cells and the relevant mechanism.Methods SMMC-7721 cells were treated with different concentrations of resveratrol.Cell proliferation was tested by cell proliferation assay and colony-forming experiment.The effect of resveratrol on cell cycle of SMMC-7721 cells was detected by flow cytometry.The activation of mTOR/p-mTOR proteins was detected by Western blot.Results Compared with control group, resveratrol inhibited SMMC-7721 cell proliferation in a dose-dependent manner(P<0.01);cell cycle detection showed that SMMC-7721 cells were arrested at G0/G1 phase by resveratrol;Westren blot assay showed that resveratrol down-regulated p-mTOR expression.Conslusion Resveratrol inhibits human SMMC-7721 cell proliferation and arrests the cell cycle at G0/G1 phase, while the inhibition mechanism may be related to activation of mTOR signaling pathway by resveratrol.关键词
白藜芦醇/肝癌/细胞周期/mTOR/p-mTOR/磷酸化Key words
resveratrol/hepatic carcinoma/cell cycle/mTOR/p-mTOR/phosphory lation分类
医药卫生引用本文复制引用
王坦,张艳群,曾永联,徐庆..白藜芦醇抑制人肝癌SMMC-7721细胞增殖并降低mTOR蛋白磷酸化水平[J].中国药理学通报,2017,33(9):1309-1314,6.基金项目
国家自然科学基金资助项目(No 81460458) （No 81460458）
