中国药理学通报2017，Vol.33Issue(9)：1320-1324,5.DOI:10.3969/j.issn.1001-1978.2017.09.025
红景天苷对局灶性脑缺血/再灌注大鼠轴突再生及Akt/GSK-3β/CRMP-2表达的影响
Salidroside regulates Akt/GSK-3β/CRMP-2expression and axonal regeneration in MCAO rats
摘要
Abstract
Aim To investigate the axonal regeneration effect of salidroside in MCAO rats and its potential mechanism.Methods Thirty-six healthy adult male Sprague-Dawley rats were randomly divided into three groups: sham, MCAO, MCAO+Sal groups.The rats were subjected to focal cerebral ischemia/reperfusion with suture-occluded method.Neurological deficit testing was performed with Zea Longa scale.The protein expression of p-Akt(Ser473), Akt, p-GSK-3β(Ser9), GSK-3β, p-CRMP-2(Thr514) and CRMP-2 in side cerebral ischemic tissues were determined using Western blot analysis.NF200 immunofluorescence staining was used to evaluate axonal regeneration.Results Compared with MCAO group,salidroside significantly improved the neurological deficit,up-regulated the protein expression of NF200,p-Akt and p-GSK-3β,and inhibited the protein expression of p-CRMP-2.Conclusions Salidroside improves neurological function recovery after focal cerebral/ischemic injury in rats,which may be associated with the up-regulation of phosphorylated Akt and GSK-3β and inhibition of phosphorylated CRMP-2,thereby promoting axonal regeneration.关键词
红景天苷/局灶性脑缺血/再灌注/轴突再生/Akt/GSK-3β/CRMP-2Key words
salidroside/local cerebral ischemia/ reperfusion/axonal regeneration/Akt/GSK-3β/CRMP-2分类
医药卫生引用本文复制引用
张小琴,苗学蒸,周丽英,许力伟,赖文芳,洪桂祝..红景天苷对局灶性脑缺血/再灌注大鼠轴突再生及Akt/GSK-3β/CRMP-2表达的影响[J].中国药理学通报,2017,33(9):1320-1324,5.基金项目
国家自然科学基金资助项目(No 81473382) （No 81473382）
福建省科技厅重点项目(No 2014Y4004) （No 2014Y4004）
福建省自然科学基金资助项目(No 2015J01685) （No 2015J01685）
福建中医药大学校管-科研创新平台专项资助项目(No X2015009-平台) （No X2015009-平台）
