中国药理学通报2017，Vol.33Issue(9)：1324-1329,6.DOI:10.3969/j.issn.1001-1978.2017.09.026
活血解毒中药下调8-OhdG表达抑制梗阻性肾病大鼠细胞凋亡
Inhibitory effects of Huoxue Jiedu decoction on cellapoptosis via down-regulating 8-OhdG in obstructive nephropathy rats
摘要
Abstract
Aim To observe the effect of eplerenone(EPL) and Chinese decoction on cell apoptosis in obstructive nephropathy rats.Methods Sixty male Wistar rats were randomly divided into sham group, UUO group, EPL group and ZY group(n=15).Except sham group, the rats in the other groups were ligated with unilateral ureteral obstruction(UUO) for renal interstitial fibrosis model.The rats were treated with eplerenone at 100 mg·kg-1·d-1 added to diet in EPL group, and orally 13.7 g·kg-1·d-1 decoction of Chinese medicine in ZY group.The kidneys were harvested on 14th day, the number of renal cell apoptosis were detected by TUNEL, and serum aldosterone and 8-OhdG were detected with radioimmunoassay and ELISA.Caspase-12, caspase-9, Bax and Bcl-2 were examined by immunohistochemistry and Western blot.Results The levels of serum aldosterone, serum and urine 8-OhdG and the number of positive apoptotic cells increased significantly in UUO rats compared with Sham group.The overexpression of caspase-9, caspase-12 and Bax and down-regulated Bcl-2 were obvious in UUO group(P<0.01).The level of 8-OhdG, expression of caspase-9, caspase-12 and Bax were down-regulated, and Bcl-2 expression was up-regulated in eplerenone and Chinese decoction treated rats(P<0.01).Conclusion Eplerenone and Chinese decoction could inhibit cell apoptosis induced by oxidative damage after UUO via caspases and(or) Bax pathway.关键词
8-羟基脱氧鸟苷/细胞凋亡/氧化损伤/依普利酮/醛固酮/肾脏纤维化/caspase-12/caspase-9/Bax/Bcl-2Key words
8-OhdG/apoptosis/oxidative damage/eplerenone/aldosterone/renal fibrosis/caspase-12/caspase-9/Bax/Bcl-2分类
医药卫生引用本文复制引用
王筝,许庆友,常奕,熊云昭,王萱,王聪慧,王淼,王香婷,孙东云,梁丽娟..活血解毒中药下调8-OhdG表达抑制梗阻性肾病大鼠细胞凋亡[J].中国药理学通报,2017,33(9):1324-1329,6.基金项目
国家自然科学基金资助项目(No 81473652) （No 81473652）
河北省自然科学基金项目(No H2016423059,H2015423009) （No H2016423059,H2015423009）
河北省高等学校科学技术研究项目(No QN2015144) （No QN2015144）
