中成药2017，Vol.39Issue(8)：1549-1554,6.DOI:10.3969/j.issn.1001-1528.2017.08.001
黄地安消胶囊对改善2型糖尿病GK大鼠胰岛素抵抗的影响
Improving effects of Huangdi Anxiao Capsules on insulin resistance in type 2 diabetic GK rats
摘要
Abstract
AIM To observe the effects of Huangdi Anxiao Capsules (Puerariae lobatae Radix,Eriobotryae Folium,Notoginseng Radix et Rhizoma,etc.) on blood glucose and insulin resistance in GK rats with type 2 diabetes mellitus (T2DM) and its possible mechanism of action.METHODS GK rats with T2DM included in the experiment were randomly divided into model group,rosiglitazone (1.44 mg/kg) group,Shenqi Jiangtang Granules (1.08 g/kg) group,Huangdi Anxiao Capsules high,medium and low (12,6,3 g/kg) group and normal control group of Wistar rats.After six weeks of consecutive administration,fasting blood glucose (FBG),serum insulin (FINS),fasting plasma glucose (FPG),and insulin resistance index (HOMA-IR) were measured in all groups.Serum biochemical indexes of (TG,TC,HDL-C,LDL-C),glycosylated hemoglobin (HbA1c),glucagon-like peptide (GLP-1) and insulin-like growth factor (IGF-1) were measured.The pancreatic tissue was obtained by routine paraffin embedding and HE staining to observe the pathological changes.RESULTS Compared with the normal group,FBG,FPG,FINS,HOMA-IR,TG,TC,HDL-C,LDL-C and HbA1c of the model group were significantly higher (P ＜0.01),and GLP-1 and IGF-1 expressions were markedly decreased (P ＜ 0.01).Compared with the model group,the levels of FBG,FPG,FINS,HOMA-IR,TG,TC,HDL-C,LDL-C and HbA1 c in Huangdi Anxiao Capsules high-,medium-and low-dose group were significantly decreased (P ＜ 0.05,P ＜0.01);GLP-1 and IGF-1 expressions were markedly increased (P ＜0.05,P ＜0.01),compared with the model group,the structure changes of pancreatic tissue in Huangdi Anxiao Capsules high-,medium-and low-dose groups largely reduced.CONCLUSION Huangdi Anxiao Capsules can reduce GK rats fasting blood glucose,insulin resistance,and its mechanism may be related to promoting the emergence and proliferation of pancreatic islet cells,improving the function of islet beta cells,and increasing insulin secretion.关键词
黄地安消胶囊/2型糖尿病/GK大鼠/胰岛素抵抗/血糖Key words
Huangdi Anxiao Capsules/type 2 diabetes/GK rat/insulin resistance/blood glucose分类
医药卫生引用本文复制引用
郭明飞,高家荣,方朝晖,姜辉,赵进东,魏良兵,单莉,韩利平,姜楠楠..黄地安消胶囊对改善2型糖尿病GK大鼠胰岛素抵抗的影响[J].中成药,2017,39(8):1549-1554,6.基金项目
国家中医药管理局中医临床研究基地业务建设第二批科研专项课题(JDZX2015126) （JDZX2015126）
安徽中医药大学青年科学研究基金(2014qn018) （2014qn018）
