中成药2017，Vol.39Issue(8)：1566-1572,7.DOI:10.3969/j.issn.1001-1528.2017.08.004
五味温通除痹胶囊对佐剂性关节炎大鼠自噬蛋白Beclin-1、LC3-Ⅱ表达的影响
Effects of Wuwei Wentong Chubi Capsules on autophagy proteins of Beclin-1 and LC3-Ⅱ in adjuvant-induced arthritis rats
摘要
Abstract
AIM To observe the effects of Wuwei Wentong Chubi Capsules (Cinnamomi Ramulus,Poria,Epimedii Folium,etc.) on autophagy proteins of Beclin-1 and LC3-Ⅱ in adjuvant-induced arthritis rats and to explore the possible mechanism of action.METHODS Sixty SD rats were randomized into six groups:normal group,model group,Wuwei Wentong Chubi Capsules (0.8,1.6,3.2 g/kg) groups and tripterygium glycosides (TPT,40 mg/kg) group.In addition to the normal group,adjuvant arthritis (AA) was induced with Freund's complete adjuvant.From the 2th day after injection of FCA,Wuwei Wentong Chubi Capsules with different doses and TPT were given by gavage once a day for 12 days.At the end of the experiment,ankle-joint samples were taken to examine the degree of AA by HE.Beclin-1 and LC3-Ⅱ mRNA were determined by real-time fluorescent quantitative PCR.Meanwhile,the protein expression levels of Beclin-1 and LC3-Ⅱ were determined by immunofluorescence histochemical staining and Western blot.RESULTS As compared with the model group,Wuwei Wentong Chubi Capsules (1.60,3.20 g/kg) not only significantly reduced histopathological injuries,but also effectively up-regulated mRNA and protein expressions of Beclin-1 and LC3-Ⅱ.CONCLUSION Wuwei Wentong Chubi Capsules has a therapeutic action on AA in rats,which might be partly associated with promoting autophagy,decreasing excessive proliferation of synovial cells,leading to the reduction of damage to articular cartilage.关键词
五味温通除痹胶囊/佐剂性关节炎/大鼠/Beclin-1/LC3-ⅡKey words
Wuwei Wentong Chubi Capsules (WWCC)/adjuvant arthritis/rat/Beclin-1/LC3-Ⅱ分类
医药卫生引用本文复制引用
姜辉,秦秀娟,万磊,刘健,高家荣..五味温通除痹胶囊对佐剂性关节炎大鼠自噬蛋白Beclin-1、LC3-Ⅱ表达的影响[J].中成药,2017,39(8):1566-1572,7.基金项目
安徽省高等学校省级自然科学研究(重点)项目(KJ2105A059) （重点）
安徽省卫生和计划生育委员会2016年省级中医药发展专项资金项目-名医特色制剂项目(2016ZYZJ01) （2016ZYZJ01）
