中成药2017，Vol.39Issue(8)：1601-1604,4.DOI:10.3969/j.issn.1001-1528.2017.08.011
三白草酮超临界CO2萃取工艺优化及三白草超临界萃取物体外抗肿瘤活性
Optimization of supercritical CO2 extraction for sauchinone and in vitro antitumor activity of Saururi Herba supercritical extract
摘要
Abstract
AIM To optimize the supercritical CO2 extraction of sauchinone and to evaluate the in vitro antitumor activity of Saururi Herba supercritical extract.METHODS With extraction pressure,extraction temperature,extraction time,entrainer (ethanol) concentration and entrainer amount as influencing factors,together with extraction rate of sauchinone as an evaluation index,orthogonal test was used for optimizing the extraction.Then MTT was applied to determining the extract's inhibitory effect on human multidrug-resistant hepatocellular carcinoma cell line (7721/Adm).RESULTS The optimal conditions were determined to be 30 MPa for extraction pressure,50 ℃ for extraction temperature,2 h for extraction time,95％ for ethanol concentration,and one time for ethanol amount,the average extraction rate of sauchinone was 0.173％.The obtained extract significantly inhibited the proliferation of 7721/Adm cells (IC50 =50.08 μg/mL),demonstrating a stronger activity than that of ethanol extract (ICs0 =150.59 μg/mL).CONCLUSION This stable and feasible method is appropriate for sauchinone extraction,and the supercritical CO2 extract from Saururi Herba shows a strong in vitro antitumor activity.关键词
三白草/三白草酮/超临界CO2萃取/体外抗肿瘤活性/7721/Adm细胞/正交试验/MTTKey words
Saururi Herba/sauchinone/supercritical CO2 extraction/in vitro antitumor activity/7721/Adm cells/orthogonal test/MTT分类
医药卫生引用本文复制引用
陈宏降,王绪楠,陈建伟,李祥..三白草酮超临界CO2萃取工艺优化及三白草超临界萃取物体外抗肿瘤活性[J].中成药,2017,39(8):1601-1604,4.基金项目
浙江省自然科学基金项目(LQ15H280006) （LQ15H280006）
浙江省教育厅科研项目(Y201226181) （Y201226181）
宁波市自然科学基金项目(2013A610271) （2013A610271）
浙江医药高等专科学校校级科研重点项目(ZPCSR2014001) （ZPCSR2014001）
