中成药2017，Vol.39Issue(8)：1620-1625,6.DOI:10.3969/j.issn.1001-1528.2017.08.016
一测多评法同时测定消癥微丸中4种柴胡皂苷
Simultaneous determination of four saikosaponins in Xiaozheng Pellets by QAMS
摘要
Abstract
AIM To establish a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous content determination of four saikosaponins in Xiaozheng Pellets (Bupleuri Radix,Cyperi Rhi-zoma,Rhei Radix et Rhizoma,etc.).METHODS The analysis of 5％ ammonia-methanol extract of this drug was performed on a Waters Xbridge C18column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 210 nm and 254 nm.With saikosaponin a as a internal standard,the relative correction factors of saikosaponins b1,b2 and c were calculated,followed by the determination of their contents.RESULTS Four saikosaponins showed good linear relationships within their own ranges (r ≥ 0.999 5),whose average recoveries were 98.08％-102.94％ with the RSDs of 0.85％-1.82％.The results obtained by QAMS approximated those obtained by external standard method.CONCLUSION This simple,precise and feasible method can be used for the quality control of Xiaozheng Pellets.关键词
消癥微丸/柴胡皂苷a/柴胡皂苷b1/柴胡皂苷b2/柴胡皂苷c/一测多评Key words
Xiaozheng Pellets/saikosaponin a/saikosaponin b1/saikosaponin b2/saikosaponin c/QAMS分类
医药卫生引用本文复制引用
夏彦铭,董迎,狄留庆,赵晓莉..一测多评法同时测定消癥微丸中4种柴胡皂苷[J].中成药,2017,39(8):1620-1625,6.基金项目
江苏省科技厅产学研前瞻性联合项目(BY2013008-01) （BY2013008-01）
江苏高效优势学科建设工程资助项目(2015) （2015）
