作物学报2017，Vol.43Issue(9)：1261-1271,11.DOI:10.3724/SP.J.1006.2017.01261
玉米ZmBRI1基因的克隆、表达及功能分析
Cloning, Expression and Functional Analysis of Brassinosteroid Receptor Gene (ZmBRI1) fromZea maysL.
摘要
Abstract
Brassinosteroids (BRs) is one of very important plant steroidal hormones that are essential in a wide variety of physio-logical processes. In this study, an encoding brassinosteroid receptor homologous gene was cloned by homology cloning from maize B73 inbred lines, and designated as ZmBRI1. Sequence analysis revealed that the full length of ZmBRI1 was 3369 bp, en-coding 1122 amino acids. Moreover, ZmBRI1 protein was localized in cell membrane by the protein subcellular localization analysis and a ubiquitously expressed receptor kinase expressed highly in young tissues. The transformation of ZmBRI1 into the Arabidopsis dwarf mutant bri1-5 restored the phenotype, including plant height, leaf morphology and pod size. Compared with bri1-5, in the transgenic plants, brassinolide (BL) inhibited significantly the root growth, propiconazole (Pcz) inhibited the hypo-cotyl growth, and the expression levels of DWF4 and CPD were decreased. Furthermore,compared with the wild type, with ABA treatment, overexpression of ZmBRI1 in the wild type increased the germination rate and plant growth, and decreased the expres-sion of ABA downstream genes RD29A,RD29B,ABI5,and RAB18. There fore,ZmBRI1is not only involved in plant morpho-genesis and BR signal transduction, but also plays a pivotal role in response to ABA signal.关键词
玉米/ZmBRI1/转基因植株/油菜素内酯/ABAKey words
Maize/ZmBRI1/Transgenic plant/Brassinosteroid/ABA引用本文复制引用
郝岭,张钰石,段留生,张明才,李召虎..玉米ZmBRI1基因的克隆、表达及功能分析[J].作物学报,2017,43(9):1261-1271,11.基金项目
本研究由引进国际先进农业科学技术计划(948计划)项目(2011-G19)资助. This study was supported by the Introduction of International Advanced Agricultural Science and Technology Program (948 Program, 2011-G19). （948计划）
