作物学报2017，Vol.43Issue(9)：1272-1279,8.DOI:10.3724/SP.J.1006.2017.01272
矮秆基因Rht_NM9在小麦株高建成中对内源激素含量的影响
Effects of Dwarf GeneRht_NM9 on Contents of Endogenous Hormone Regu-lating Plant Height of Common Wheat
摘要
Abstract
A dwarf wheat mutant (NM9) with additional tillers and elongated spikes was obtained by treating NAU9918 seeds with ethyl methanesulfonate (EMS). The decreased plant height in NM9 was controlled by a novel dwarf gene Rht_NM9. The endogenous plant hormones play important roles in regulating plant height of common wheat. To understand the dwarfing mecha-nism of Rht_NM9 and the relationship between endogenous hormone contents and plant height, measured contents of endogenous gibberellic acid (GA), auxin (IAA), abscisic acid (ABA) and zeatin riboside (ZR) in internodes of NM9 and NAU9918 at different stages by enzyme-linked immunosorbent assays (ELISA). Our study indicated that contents of GA and ABA in NM9 were sig-nificantly higher than there in NAU9918, and ZR content in NM9 was significantly lower than that in NAU9918 at both booting and heading stages. Nevertheless, no difference of IAA content was observed between the mutant and the wild-type. In addition, the GA/ABA ratio in internodes of the mutant was significantly higher than that of the wild-type, however, the ratios of IAA/ABA, (IAA + GA)/ABA and ZR/ABA were significantly lower than there of the wild-type. All these results indicated that plant height in wheat was regulated by multiple hormones. Plant height would be inhibited with increasing content of endogenous ABA and de-creasing ratios of IAA/ABA and ZR/ABA in wheat.关键词
小麦/株高/突变体/内源激素Key words
Wheat/Plant height/Mutant/Endogenous hormones引用本文复制引用
卢媛,崔超凡,胡平,陈佩度,沈雪芳,韩晴,王义发,邢莉萍,曹爱忠..矮秆基因Rht_NM9在小麦株高建成中对内源激素含量的影响[J].作物学报,2017,43(9):1272-1279,8.基金项目
本研究由中央高校基本科研业务费专项(KYZ201601, KYYJ201602, KYZ201401)资助. This study was supported by the Fundamental Research Funds for the Central Universities (KYZ201601, KYYJ201602, KYZ201401). （KYZ201601, KYYJ201602, KYZ201401）
