作物学报2017，Vol.43Issue(9)：1280-1289,10.DOI:10.3724/SP.J.1006.2017.01280
甘蓝型油菜茎秆菌核病抗性与木质素含量及其单体G/S的相关性分析及QTL定位
Correlation Analysis of Sclerotinia Resistance with Lignin Content and Mono-mer G/S and Its QTL Mapping in Brassica napus L.
摘要
Abstract
Sclerotinia sclerotiorumis a fungal pathogen causing disease in a wide range of plants, resulting in serious damage in crop production. The detached stem inoculation assay of RIL and F2 populations at final flowering stage was conducted, near in-frared (NIR) spectroscopy was used to measure lignin content and monomer G/S in the stem, and correlation analysis and QTL mapping for these traits were performed. The lesion size of the RIL had a significantly negative correlation with lignin content, with a correlation coefficient at –0.348 and –0.286 in 2013 and 2014, respectively. The monomer G/S was significantly correlated with lesion size in the RIL population, and the correlation coefficient at 0.198 and 0.167 in 2013 and 2014, respectively. The le-sion size of F2 in 2014 was significantly and negatively correlated with lignin content in the stem, with a correlation coefficient at –0.306. The cross sections of resistant and susceptible plants from F2:3family were stained with phloroglucinol-HCl, showing that the content of lignin was significantly lower in the less resistant materials than in more resistant plants. According to the high density SNP genetic maps and composite interval mapping, a total of 18 QTLs were identified, which were located on A04, A05, A06, A08, C01, C03, C04, C06, and C07 chromosomes, with the explained phenotypic variation by individual QTL ranging from 2.38% to 12.05% for nine QTLs of lesion size, from 2.03% to 13.75% for three QTLs of lignin content, and from 2.06% to 8.66% for six QTLs of monomer G/S. The research results provide some new insights for the Sclerotinia resistance breeding in B. napus.关键词
甘蓝型油菜/菌核病/相关性分析/数量性状位点/木质素/单体G/SKey words
Brassica napus/Sclerotinia sclerotiorum/Correlation/QTL/Lignin/Monomer G/S引用本文复制引用
陈雪萍,荆凌云,王嘉,荐红举,梅家琴,徐新福,李加纳,刘列钊..甘蓝型油菜茎秆菌核病抗性与木质素含量及其单体G/S的相关性分析及QTL定位[J].作物学报,2017,43(9):1280-1289,10.基金项目
本研究由国家自然科学基金项目(31371655)和重庆市科委(cstc2016shmszx80083)项目资助. This study was supported by the National Natural Science Foundation of China (31371655) and the Science and Technology Committee of Chongqing (cstc2016shmszx80083). （31371655）
