作物学报2017，Vol.43Issue(9)：1290-1299,10.DOI:10.3724/SP.J.1006.2017.01290
木薯块根有色体分离及其蛋白质组学的研究
Chromoplast Isolation and Its Proteomic Analysis from Cassava Storage Roots
摘要
Abstract
Chromoplasts are the sites to store carotenoids and regulate a variety of physiological and biochemical process in the storage roots of cassava (Manihot esculenta Crantz). In the present study, it was found that Percoll density gradient centrifugation was suitable for isolating the chromoplasts from cassava storage roots. Rich and intact chromoplasts were located in 40% to 50% layer of Percoll and the expression level ofVdac1, a mitochondrial marker, was the lowest and the expression level ofRbcL, a plastid marker, was the highest compared with other layers using Western blot. Thirty-four differentially expressed proteins were detected in SC9, in which 17 were up-regulated, and the others were down-regulated. The differential proteins related to carbohy-drate and energy metabolism accounted for the highest proportion. The STRING protein-protein interaction network showed that Enolase 2 and Elongation Factor were the hub proteins, which play the key roles in the whole regulatory network. Quantitative analysis by qRT-PCR confirmed theEnolase 2expression was more significantly up-regulated in the high carotenoid cassava variety than in the low carotenoid cassava SC6068. These two proteins may be the key points for affecting the carotenoid content between SC9 and SC6068.关键词
木薯块根/有色体蛋白质/分离/蛋白质组Key words
Cassava storage root/Chromoplast protein/Isolation method/Proteomics引用本文复制引用
邓昌哲,姚慧,安飞飞,李开绵,陈松笔..木薯块根有色体分离及其蛋白质组学的研究[J].作物学报,2017,43(9):1290-1299,10.基金项目
本研究由国家自然科学基金项目(31271776)和海南省高层次创新创业人才基金项目(2012-2016)资助. The study was supported by the National Natural Science Foundation of China (31271776) and the Initial Fund of High-level Creative Talents in Hainan Province (2012–2016). （31271776）
