作物学报2017，Vol.43Issue(9)：1308-1318,11.DOI:10.3724/SP.J.1006.2017.01308
不同脱水剂对杂交水稻制种种子质量及基因表达的影响
Effects of Different Dehydrating Agents on Seed Quality and Gene Expression in Hybrid Rice Seed Production
摘要
Abstract
Seed dehydration is very important in hybrid rice seed production. For seed production safety, dehydrating agents are used in this process because natural dehydration is slow and easily affected by rainy weather, which often results in seed late ma-ture of and pre-harvest sprouting. In this study, the effects of different dehydrating agents on seed dehydration and quality were compared using hybrid rice variety "Y Liangyou 689". The eleven dehydrating agents tested all accelerated seed dehydration. Particularly, agents No.7 and No.9 resulted in seed moisture content at five days after spray decreased by 4.6% and 3.6%, respec-tively, and had no negative effects on 1000-grain weight, seed germination and seedling growth. Moreover, both agents signifi-cantly increased the contents of soluble protein, soluble sugar, ABA and GA3 in seeds. After storage at room temperature for six months, agents No.7, No.8, and No.9 had no negative effects on seed germination and seedling growth as compared to the control. There were no significant differences in soluble protein content,α-amylase activity and ABA content among agents No.7, No.9 and the control. Agent No.7 was better in lowering seed moisture content than No.9, suggesting its potential use in Y Liangyou 689 seed production. Interestingly, agents No.7, No.8, and No.9 inducedOsNECD1andOsNCED2 expressions and reduced OsGA2ox1 expression in seeds before storage. After six-month storage, the expressions ofOsNECD1 andOsNCED2 decreased and the expression ofOsCYP707A5 andOsGA2ox1 increased in seeds treated with agents No.7, No.8, and No.9. This result indi-cate that the effects of dehydrating agents on ABA biosynthesis genes and GA3 catabolism genes in seeds weakened during stor- age and the high expression level ofOsCYP707A5 resulted in the decline of ABA content, which might be a main reason for better seed germination.关键词
杂交水稻/脱水剂/种子质量/成熟脱水/基因表达Key words
Hybrid rice/Dehydration agent/Seed quality/Mature dehydration/Gene expression引用本文复制引用
沈杭琪,胡伟民,林程,关亚静,刘宏友,安建宇,胡晋..不同脱水剂对杂交水稻制种种子质量及基因表达的影响[J].作物学报,2017,43(9):1308-1318,11.基金项目
本研究由国家公益性行业(农业)科研专项(201203052),浙江省科技厅项目(2013C02005, 2013C32023),浙江省自然科学基金项目(LZ14C130002, LY15C130002)和江苏省现代作物生产协同创新中心项目资助. This study was supported by the China Special Fund for Agro-scientific Research in the Public Interest (201203052), the Science and Tech-nology Department of Zhejiang Province (2013C02005, 2013C32023), the Natural Science Fundation of Zhejiang Province (LZ14C130002, LY15C130002), and the Jiangsu Collaborative Innovation Center for Modern Crop Production. （农业）
