作物学报2017，Vol.43Issue(9)：1337-1346,10.DOI:10.3724/SP.J.1006.2017.01337
甘蔗与抗旱性相关差异蛋白质组分析
Analysis of Differential Proteome in Relation to Drought Resistance in Sugarcane
摘要
Abstract
Drought stress is a major restraint in sugarcane production in China. Proteomic study in relation to drought stress pro-vides valuable information in drought resistant breeding of sugarcane. In this study, the drought-resistant sugarcane variety, F172, and the drought-sensitive variety, YL6, were used in a pot experiment for differential proteome analysis. Seedlings of both varie-ties were exposed to severe drought stress for seven days and the leaf proteins were separated and analyzed using 2-DE technique and PDQuest software. From the protein profiles of F172 and YL6, 28 and 20 differential protein spots were detected between normal-irrigation and drought-stress treatments, respectively, including up- and down-regulated proteins and new protein spots. The differential proteins varied across the two varieties. Using MALDI-TOF-TOF/MS, 18 and 14 amino acid sequences were identified from YL6 and F172, respectively, and they were in eight function categories. In YL6, the 18 proteins consist of two participating in oxygen radical scavenging, six participating in photosynthesis, one participating in cell growth and division, six participating in basic metabolisms, two participating in protective response, and one unknown in function. In F172, the 14 proteins consist of one participating in oxygen radical scavenging, two participating in photosynthesis, two participating in cell growth and division, four participating in basic metabolisms, two participating in information transfer, one participating in protein processing, and one unknown in function. A drought-induced protein of 22 kDa was in high level in F172 but absence in YL6. These results indicate that protein compositions under drought stress are highly different in sugarcane varieties with different drought resistance and the differential proteins might give a hint to drought-resistant mechanism.关键词
甘蔗/水分胁迫/蛋白质组/差异表达/抗旱性Key words
Sugarcane/Water stress/Proteome/Differential expression/Drought resistance引用本文复制引用
DO Thanh-Trung,李健,张风娟,杨丽涛,李杨瑞,邢永秀..甘蔗与抗旱性相关差异蛋白质组分析[J].作物学报,2017,43(9):1337-1346,10.基金项目
本研究由国家高技术研究发展计划项目(863计划)(2013AA102604), 广西八桂学者、特聘专家专项(2013), 国家现代农业产业技术体系广西甘蔗创新团队专项(gjnytxgxcxtd-03-01)和广西甘蔗遗传改良重点实验室项目(12-K-05-01)资助. This study was supported in part by the National High Technology Research and Development Program of China (863 program) (2013AA102604), the Special Funds for Bagui Scholars and Distinguished Experts in Guangxi (2013), the Guangxi Sugarcane Innovation Team of National Agricultural Industry Technology System (gjnytxgxcxtd-03-01), and the Guangxi Key Laboratory of Sugarcane Genetic Improvement (12-K-05-01). （863计划）
