作物学报2017，Vol.43Issue(9)：1410-1414,5.DOI:10.3724/SP.J.1006.2017.01410
玉米不同组织器官谷氨酰胺合成酶同工酶表达差异及聚合方式
Differential Expression and Assembly Mode of Glutamine Synthetase Isoen-zymes in Different Tissues and Organs of Maize
摘要
Abstract
Glutamine synthetase (GS) is a key enzyme in nitrogen assimilation and recycling in cereals. In this study, the expres-sion characteristics of GS isoenzymes in different tissues and organs of maize in grain-filling period were analyzed, and the as-sembly of GS isoenzymes were indentified. The GS isoforms expressed differentially in different organs were shown by West-ern-blot obviously; GS1 with a molecular weight of about 40 kD expressed highly in all tissues, and GS1 with a molecular weight of about 39 kD was merely expressed in the node of ear position and pedical, and GS2 with a molecular weight of about 44 kD was weakly expressed in the photosynthtic tissue such as leaf. With a modified blue naive PAGE (BNE) technique and in-gel ac-tivity analysis, the size of GS holoenzyme was calibrated; combined the 2-D gel with western-blot analysis, the subunits composi-tion of GS isoenzymes were identified. Three GS isoenzymes with different sizes were identified in maize. GS2 holoenzyme was about 460 kD and likely a decamer, GS1 holoenzyme existed two kinds of assembly state, one was about 410 kD and likely a decamer, another was about 240 kD and more likely a pentamer; therefore, the expression of GS isoenzymes exists diversity in maize.关键词
玉米/谷氨酰胺合成酶(GS)/表达/BNE/聚合方式Key words
Maize/Glutamine synthetase (GS)/Expression/Blue native PAGE (BNE)/Assembly引用本文复制引用
王小纯,张浩然,韦一昊,贾喜婷,谷明鑫,马新明..玉米不同组织器官谷氨酰胺合成酶同工酶表达差异及聚合方式[J].作物学报,2017,43(9):1410-1414,5.基金项目
本研究由国家重点研发计划项目(2016YFD0300205)和小麦玉米作物学国家重点实验室(39990047)资助. The study was supported by the National Key Research and Development Program of China (2016YFD0300205) and State Key Laboratory of Wheat and Maize Crop Science (39990047). （2016YFD0300205）
