北京中医药大学学报2017，Vol.40Issue(8)：635-640,6.DOI:10.3969/j.issn.1006-2157.2017.08.004
补肾调经方、逍遥丸对促排卵小鼠排卵前卵巢卵母细胞分泌因子表达的影响
Effects of Bushen Tiaojing Fang and Xiaoyao Wan on the expression of o-ocyte secreted factors in mouse ovary before ovulation
摘要
Abstract
Objective To investigate the possible mechanisms of ovulation induced by supplementing kidney and smoothing liver by observing the effects of Bushen Tiaojing Fang(Kidney-tonifying Menstrua-tion-regulating Formula,BSTJF)and Xiaoyao Wan(Free Wanderer Pill,XYW)on the expression of growth differentiation factor-9 (GDF-9),bone morphogenetic protein (BMP-15 )mRNA and its protein expression.Methods Immature female mice were randomly divided into normal control group,kidney-tonifying(BSTJF)group and liver-soothing(XYW)group.All groups received intraperitoneal injection of 5 IU pregnant mare serum gonadotrophin(PMSG)at day 3 and 5 IU human chorionic gonadotropin(hCG) at day 5;mice in kidney-tonifying group and liver-soothing group received oral gavage of BSTJF (18.70 g/kg BSTJFⅡ from day 1 to day 3;28.57 g/kg BSTJFⅢ from day 4 to day 5)or XYW (2.34 g/kg XYW from day 1 to day 3;1.17 g/kg XYW from day 4 to day 5)respectively.GDF-9,BMP-15mRNA and its protein expression in ovary were measured with real-time fluorescence quantitative PCR,Western blot,and immunohistochemical analysis at the 0,8,12 hours after hCG injection.Results Compared with the normal control group,BSTJF and XYW increased the expressions of GDF-9,BMP-15mRNA and its protein at 8 and 12 hours after hCG injection(P <0.05).Conclusion Bushen Tiaojing Fang and Xiaoyao Wan may induce ovulation by promoting proliferation of ovarian granular cells,expansion of cu-mulus oophorus,and maturity of oocyte through increasing the expression of GDF-9,BMP-15mRNA and its protein.关键词
补肾调经方/逍遥丸/排卵/小鼠Key words
Bushen Tiaojing Formula/Xiaoyao Pill/ovulation/mice分类
医药卫生引用本文复制引用
段彦苍,宋翠淼,贺明,杜惠兰,刘亚华..补肾调经方、逍遥丸对促排卵小鼠排卵前卵巢卵母细胞分泌因子表达的影响[J].北京中医药大学学报,2017,40(8):635-640,6.基金项目
国家自然科学基金项目(No.81303002),河北省自然科学基金项目(No.H2013206117),河北省教育厅项目(No.Q20122008) National Natural Science Foundation of China (No.81303002),Natural Science Foundation of Hebei Province (No.H2013206117),Hebei Provincial Department of Education (No.Q20122008). （No.81303002）
