北京中医药大学学报2017，Vol.40Issue(8)：641-645,5.DOI:10.3969/j.issn.1006-2157.2017.08.005
不同光制下小鼠下丘脑视交叉上核Clock mRNA昼夜表达特点及温胆汤的干预作用
Experimental study on the different day-night expression of hypothalamus SCN Clock mRNA in mice under different light systems and Wendan Tang's intervention
摘要
Abstract
Objective To observe the characteristics of the hypothalamus SCN Clock mRNA expression in mice under four timing points in a day and light condition and the effects of Wendan Tang (Gallbladder-Warming Decoction,WDT).Methods 160 male KMmice of 2 ～3 months were randomly divided into normal group (6:00—18:00 light),dark group (24 h dark),model group (24 h light),WDT group, and melatonin group(n =32 in each group).Zeitgeber time (ZT)and Circadian time (CT)were used to indicate natural light and dark timing points respectively At the last day of the 12 th week,8 mice from each group were randomly selected and sacrificed at natural light timing points of 6 a.m.(ZT0 /CT0), 12 (ZT6 /CT6),18 (ZT12 /CT12),and 24 (ZT18 /CT18).The concentration of melatonin in ser-um was measured by using enzyme-linked immunosorbent assay (ELISA).The expression of SCN Clock mRNA in mouse hypothalamus was detected by using polymerase chain reaction (PCR) method. Results Serum melatonin:The melatonin level at ZT18 of mice in the normal group was the highest (P<0.05)among all groups.Serum melatonin level in mice of the dark group at CT0 was lower than those at CT18 and CT12 (P <0.05).There were no differences in the melatonin levels between four timing points in mice of the model group (P >0.05).Serum melatonin levels in mice of the WDT group were lower at ZT0 and ZT6 than that at ZT18 (P <0.05)while these values at ZT0,ZT6 and ZT12 were lower than that at ZT18 in mice of the melatonin group (P <0.05).Expression of SCN Clock mRNA in mice hypothalamus:The expression of SCN Clock mRNA was lower at ZT18 than that at ZT6 (P <0.05).No differences were found at the four timing points in mice of the dark group (P >0.05).The expression levels in mice of the model group were lower at ZT0 and ZT12 compared with ZT18 (P <0.05);also lower at ZT0 and ZT12 compared with ZT 6 (P <0.05).The expression levels in mice of WDT and me-latonin groups at ZT18 were lower than those at ZT6 (P <0.05).Conclusion WDT is rich in melato-nin and could adjust biological clock rhythm disorder and reduction of melatonin due to continuous light.关键词
温胆汤/褪黑素/生物钟/基因/光照/天人相应/小鼠Key words
wendan tang/melatonin/biological clock/gene/light/correspondence between man and universe/mouse分类
医药卫生引用本文复制引用
杨阳,张明泉,蒲晓田,谭东宇,马淑然..不同光制下小鼠下丘脑视交叉上核Clock mRNA昼夜表达特点及温胆汤的干预作用[J].北京中医药大学学报,2017,40(8):641-645,5.基金项目
河北省教育厅高等学校科学技术研究项目(No.QN2015043),河北中医学院博士基金课题(No.BSZ2014003),国家重点基础研究发展计划"973"计划项目(No.2001CB505101) Science and Technology Research Project of College and University,funded by Hebei Province Department of Education(No.QN2015043),Na-tional Key Basic Research and Development Program (973)Program (No.2001CB505101) （No.QN2015043）
