北京中医药大学学报2017，Vol.40Issue(8)：684-692,9.DOI:10.3969/j.issn.1006-2157.2017.08.011
基于酚类特征图谱的山楂质量表征关联分析研究
Quality representation and correlation analysis of Shanzha based on phe-nolic characteristic spectrum of medical system
摘要
Abstract
Objective To establish quality representation and correlation analysis method based on the spectrum of medicinal system of Shanzha(Fructus Crataegi,Chinese hawthorn fruit),so as to evaluate quality of Shanzha decocting pieces effectively and accurately.Methods HPLC-PDA method was ap-plied to characterize quality of spectrum of Shanzha medicinal system;quality of 11 batches of Shanzha decocting pieces were characterized based on the number and chemical type of peaks on characteristic spectrum.The content of characteristic index components,protocatechuic acid,epicatechin,chlorogeni-cacid,phenolic acids (represented by protocatechuic acid and chlorogenic acid)and flavonoids (repre-sented by hyperoside and epicatechin)were characterized by indexes in characteristic spectrum.Correla-tion analysis of quality and quantity of Shanzha was then performed with reference to the standard refer-ence pieces.Results As the reference substance,characteristic spectrum of batch 1 contains 13 charac-teristic peaks,7 peaks of flavonoids and 6 peaks of phenolic acids.All characteristic peaks appeared on the chromatograms of 11 batches of Shanzha.Batch 5,10,8,2,6 had more effective index ingredients. After comprehensive evaluation,there was a high correlation between batch 6,4,3,7,8,2 and standard reference pieces.The quality of batch 5,10,8,2,7,and 3 ranked on the top among all samples.Con-clusion HPLC method to characterize quality and quantity of characteristic spectrum of 11 batches of Shanzha is simple and accurate.The evaluation mode of quantity and quality and their correlation based on Shanzha medical system,including systemic correlation and application validity,can evaluate the quality of hawthorn pieces effectively and accurately.关键词
山楂/特征图谱/质量评价/关联分析Key words
Shanzha/characteristic spectrum/quality evaluation/correlation analysis分类
医药卫生引用本文复制引用
赵博,李倩,张元元,吕珊,陈梦杰,石任兵,田恒康,孙道涵,申立峰,孔静,路东波,李文霞,武秋红,刘璐..基于酚类特征图谱的山楂质量表征关联分析研究[J].北京中医药大学学报,2017,40(8):684-692,9.基金项目
十二五国家科技支撑计划项目(No.2012BAI29B06),北京中医药大学重点学科开放课题(No.2013-2DXKKF-23) National Sci-Tech Support Plan of the 12th Five-Year Plan (No.2012BAI29B06) （No.2012BAI29B06）
