重庆医学2017，Vol.46Issue(23)：3177-3179,3183,4.DOI:10.3969/j.issn.1671-8348.2017.23.003
白细胞介素-17对人牙周膜成纤维细胞表达RANKL、OPG的影响
Effects of different concentration of IL-17 on the expression of RANKL and OPG in human periodontal ligament fibroblasts
摘要
Abstract
Objective To study the effects of different concentration of interleukin(IL)-17 on the expression of receptor activator of nuclear factor-κB ligand (RANKL) and osteoprogeterin (OPG) in human periodontal ligament fibroblasts (HPDLF) and explore the relationship between IL-17 and orthodontic root resorption.Methods HPDLF cell line was established through the tissue pieces culture method in vitro.HPDLF were stimulated by IL-17 with five different concentrations(0,5,10,20,40 ng/mL) for 24 h.The expression level of mRNA and protein of RANKL and OPG in HPDLF were detected by RT-PCR and ELISA,respectively.Results HPDLF expressed RANKL and OPG in 0 ng/mL group.The expression amount of mRNA and protein of RANKL in HPDLF was positive correlation with the concentration of IL-17 in 0 to 20 ng/mL group.The expression amount of mRNA and protein of OPG was negative correlation with the concentration of IL-17 in 5 to 20 ng/mL group.The ralative RANKL/OPG ratio of mRNA and protein were positive correlation with the concentration of IL-17 in 0 to 20 ng/mL group.Conclusion HPDLF expresses RANKL and OPG in the absence of IL-17.IL-17 enhances the expression of RANKL and inhibits the expression of OPG in HPDLF.关键词
牙用膜/成纤维细胞/白细胞介素17/RANK配体/骨保护素Key words
periodontal ligament/fibroblasts/IL-17/RANK ligand/osteoprotegerin分类
医药卫生引用本文复制引用
李琳娟,黎敏,彭娟敏,康娜..白细胞介素-17对人牙周膜成纤维细胞表达RANKL、OPG的影响[J].重庆医学,2017,46(23):3177-3179,3183,4.基金项目
国家自然科学基金资助项目(81360170). （81360170）
