重庆医学2017，Vol.46Issue(23)：3180-3183,4.DOI:10.3969/j.issn.1671-8348.2017.23.004
siRNA干扰膜联蛋白AI表达对甲状腺乳头状癌TPC-1细胞凋亡的影响
Influnence of siRNA interfering Annexina A1 expression on apoptosis of papillary thyroid carcinomar TPC-1 cells
摘要
Abstract
Objective To investigate the effect of ANX A1 on the biological characteristics of papillary thyroid carcinom cells by interfering with the expression of Annexina A1 (ANX A1) in papillary thyroid carcinoma cells through small interfering RNAs (siRNA).Methods The designed highly efficient siRNA was used to conduct the specific interfence on ANXA1 expression in the papillary thyroid carcinoma TPC-1 cells.The effect of ANXA1 on TPC-1 apoptosis in PTC was observed by flow cytometry.Results The designed siRAN could efficiently inhibit the expression of ANXA1 mRNA in PTC,enhanced the cell apoptosis in TPC-1 cells in vitro.Conclusion siRNA can interfere with the expression of ANXA1 and promote the apoptosis of papillary thyroid carcinoma which suggesting that ANXA1 may be an important biological target for the treatment of papillary thyroid carcinoma.关键词
甲状腺肿瘤/乳头状瘤/膜联蛋白A1/TPC-1细胞/RNA,小分子干扰/凋亡Key words
thyroid neoplasms/papilloma/annexin A1/TPC-1 cells/RNA, small Interfering/apoptosisec分类
医药卫生引用本文复制引用
钟雪梅,林一民,周源,邓世山,丁浩..siRNA干扰膜联蛋白AI表达对甲状腺乳头状癌TPC-1细胞凋亡的影响[J].重庆医学,2017,46(23):3180-3183,4.基金项目
国家自然科学基金资助项目(81172496). （81172496）
