重庆医学2017，Vol.46Issue(23)：3184-3188,5.DOI:10.3969/j.issn.1671-8348.2017.23.005
miR-375通过抑制KLF4促进前列腺癌细胞的迁移和侵袭
miR-375 promotes prostate cancer cell migration and invasion by targeting KLF4
潘恩山 1李煜罡 1朱晓光1
作者信息
- 1. 南方医科大学中西医结合医院泌尿外科,广州 510315
- 折叠
摘要
Abstract
Objective To explore the expression,role and mechanism of miR-375 in prostate cancer (PCa) cells.Methods PCa cells were cultured and transfected with plasmid,the migration and invasion of PCa were detected by Transwell;the expression of miR-375 and KLF4 mRNA were detected by qPCR;the expression of KLF4 were detect by Western blot;the potential target genes of miR-375 were analyzed by bioinformatics and verified by dual luciferase report.Results The expression of miR-375 were significantly up-regulated in the PCa;Inhibited the expression of miR-375 could significantly inhibit the migration and invasion of PCa cells.KLF4 was the potential target genes of miR-375,which verified through bioinformatics analysis and dual luciferase report.The expression of KLF4 were significantly down-regulated in the PCa.Inhibited the expression of miR-375 could significantly up-regulated the expression of KLF4.Inhibited the KLF4 expression was able to reverse the suppressive effect miR-375 has on the migration and invasion of PCa cells.Conclusion miR-375 promotes the migration and invasion of PCa via inhibiting the expression of KLF4 and play the oncogene role.MiR-375 can be used as therapeutic targets for PCa,and provide a new direction for the treatment of PCa.关键词
miR-375/KLF4/前列腺肿瘤/细胞运动/肿瘤侵润Key words
microRNA 375/KLF4/prostatic neoplasms/cell movement/neoplasm invasiveness分类
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潘恩山,李煜罡,朱晓光..miR-375通过抑制KLF4促进前列腺癌细胞的迁移和侵袭[J].重庆医学,2017,46(23):3184-3188,5.
