重庆医学2017，Vol.46Issue(23)：3189-3191,3.DOI:10.3969/j.issn.1671-8348.2017.23.006
槲皮素对TNF-α诱导的乳鼠心肌成纤维细胞增殖及P38MAPK、HMGB1蛋白表达的影响
Effects of quercetin on proliferation and expression of P38MAPK and HMGB1 protein in neonatal rat cardiac fibroblasts induced by TNF-α
摘要
Abstract
Objective To study the effect of quercetin on proliferation and expression of P38MAPK and HMGB1 protein in neonatal rat cardiac fibroblasts induced by TNF-α.Methods Purified cardiac fibroblasts were obtained by trypsin digestion and differential adherence method.The proliferation of cardiac fibroblasts was detected by MTT assay.The expression of P38MAPK and HMGB1 protein was detected by Western blot.Results Quercetin had no effect on the proliferation of cardiac fibroblasts in the basal state but inhibited the proliferation of cardiac fibroblasts induced by TNF-α,and the A value of each group was increased with the increase of quereetin concentration(P＜0.05).The exspression of P38MAPK、HMGB1 were decresed with the icrease of quercetin.Conclusion Quercetin may inhibit the proliferation of cardiac fibroblasts induced by TNF-α.The mechanism may be inhibit the expression of HMGB1 through P38MAPK signaling pathway.关键词
槲皮素/成纤维细胞/心肌/心室重构/高迁移率族蛋白类/丝裂素活化蛋白激酶Key words
quercetin/fibroblasts/myocardiu/ventricular remodeling/high mobility group/proteins/mitogen-activated protein kinase分类
医药卫生引用本文复制引用
章春,冯健,李家富..槲皮素对TNF-α诱导的乳鼠心肌成纤维细胞增殖及P38MAPK、HMGB1蛋白表达的影响[J].重庆医学,2017,46(23):3189-3191,3.基金项目
四川省科技厅国际合作项目(2012HH0011). （2012HH0011）
