重庆医学2017，Vol.46Issue(23)：3234-3237,4.DOI:10.3969/j.issn.1671-8348.2017.23.021
HPLC-MS/MS法在骨髓移植患者全血CSA及AM1浓度测定中的应用
The application of HPLC-MS/MS method in the determination of whole blood concentration of Cyclosporine A and AM1 in bone marrow transplant patient
王磊 1刘红星 1孙文利1
作者信息
- 1. 河北燕达陆道培医院临床药理室,河北廊坊 065201
- 折叠
摘要
Abstract
Objective To develop a HPLC-MS/MS methodfor determination of cyclosporin A(CSA)and AM1 in bone marrow transplant patient,and explore the relationship of CSA and its main metabolite AM1 within individual and between individuals,and provide reliable basis for clinical rational use of monitoring in CSA blood drug concentration.Methods CSD was used as internal standard,and whole blood samples were treated with internal standard methanol precipitated protein.The column was Ultimate XB-C18 with a column temperature of 65 ℃ and the mobile phase was eluted with 0.1％ of formic acid and 2 mmol/L ammonium acetate in water and methanol containing 0.1％ formic acid.The mass spectrometry was detected by electrospray ion trap positive ion mode,MRM scanning,monitoring CSA 1219.9 to 1203.1 m/z,AM1 1236.1 to 1219.1 m/z,CSD 1234.0 to 1217.0 m/z.Results The concentration of CSA was linear in the range of 16 to 1600 ng/mL,Y=0.0143X+0.0213(r=0.9976).The concentration of AM1 was linear in the range of 10～1000 ng/mL,Y=0.00363X-0.00528(r=0.9973).The ratio of CSA to AM1 (AM1/CSA) between individual ranged from 32％ to 356％.The ratio of CSA to AM1 within indiviolual(AM1/CSA) ranged from 27 ％ to 147 ％.Conclusion HPLC-MS/MS method for the simultaneous detection of CSA and AM1 was established.The variation of CSA exists in the bone marrow transplant patient between individuals and within individual;the HPLC-MS/MS method can be used for monitoring of whole blood concentration of CSA in bone marrow transplant patients.关键词
环孢素A/AM1/液质联用/药物监测Key words
cyclosporin A/AM1/HPLC-MS/MS/drug monitoring分类
医药卫生引用本文复制引用
王磊,刘红星,孙文利..HPLC-MS/MS法在骨髓移植患者全血CSA及AM1浓度测定中的应用[J].重庆医学,2017,46(23):3234-3237,4.
